Anti-Human CD68 (Y1/82A)

CD68 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra)

Conjugate

Unconjugated

Publications

7

CloneNo.

Y1/82A

Cat no : 65187-1-Ig

Synonyms

CD68, CD68 molecule, GP110, Macrosialin, SCARD1, Y1/82A

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Validation Data Gallery

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  IF Staining of THP-1 using 65187-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD68 antibody (65187-1-Ig, Clone: Y1/82A ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  FC experiment of human PBMCs using 65187-1-Ig

  1X10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD68 (65187-1-Ig, Clone:Y1/82A) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

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Tested Applications

• Positive IF/ICC detected in: THP-1 cells
• Positive FC (Intra) detected in: human PBMCs
• Positive FC detected in: human PBMCs

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• FC: See 1 publications below

Product Information

65187-1-Ig targets CD68 in WB, IHC, IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Lysosomal contents of lung macrophages
• Full Name: CD68 molecule
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: BC015557
• Gene Symbol: CD68
• Gene ID (NCBI): 968
• RRID: AB_2918458
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Publications

Species	Application	Title
mouse	IF	Int Immunopharmacol; Diosgenin alters LPS-induced macrophage polarization by activating PPARγ/NF-κB signaling pathway; Authors - Xingchen Xiang
mouse	IF	Phytomedicine; Koumine regulates macrophage M1/M2 polarization via TSPO, alleviating sepsis-associated liver injury in mice; Authors - Gui-Lin Jin
mouse	FC	Int Immunopharmacol; Macrophages regulates the transition of pericyte to peritoneal fibrosis through the GSDMD/IL-1β axis; Authors - Qiuyuan Shao
human	IF	Hepatology; Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis; Authors - Xianzhi Liu
mouse	IF	Exp Neurol; Microglia trigger the structural plasticity of GABAergic neurons in the hippocampal CA1 region of a lipopolysaccharide-induced neuroinflammation model; Authors - Juan Chen
rat	WB,IHC	Int Immunopharmacol; Inhibition of aquaporin-9 ameliorates severe acute pancreatitis and associated lung injury by NLRP3 and Nrf2/HO-1 pathways; Authors - Jiawei Chen