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FITC Plus Anti-Human CD68 (Y1/82A)

CD68 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human and More (1)

Applications

IF, FC (Intra)

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

Y1/82A

Cat no : FITC-65187

Synonyms

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD68 (Y1/82A) (FITC-65187)

    FC experiment of human PBMCs using FITC-65187

    1X10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 (FITC-65187, Clone:Y1/82A). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD68 (Y1/82A) (FITC-65187)

    FC experiment of human PBMCs using FITC-65187

    1X10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 (FITC-65187, Clone:Y1/82A). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. Monocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

FITC-65187 targets CD68 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Lysosomal contents of lung macrophages
Full Name CD68 molecule
Calculated Molecular Weight 37 kDa
GenBank Accession NumberBC015557
Gene Symbol CD68
Gene ID (NCBI) 968
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Publications

SpeciesApplicationTitle
humanIF

Cell Rep Methods

Modeling alcohol-associated liver disease in humans using adipose stromal or stem cell-derived organoids

Authors - Guoyun Bi
View Article