PE-conjugated ASGR1 Polyclonal antibody

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• KD/KO Validated

ASGR1 Polyclonal Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC

Conjugate

PE Fluorescent Dye

Cat no : PE-11739

Synonyms

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

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Validation Data Gallery

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  FC experiment of HepG2 using PE-11739

  1x10^6 HepG2 cells were surface stained with 0.2 ug PE Asgr1 Polyclonal Antibody (PE-11739)(red), or 0.2 ug PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9) (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-11739 targets ASGR1 in FC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASGR1 fusion protein Ag2310
• Full Name: asialoglycoprotein receptor 1
• Calculated Molecular Weight: 291 aa, 33 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC032130
• Gene Symbol: ASGR1
• Gene ID (NCBI): 432
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.