CoraLite®555-conjugated ASGR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ASGR1 Polyclonal Antibody for IF-P, FC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC

Conjugate

CoraLite®555 Fluorescent Dye

Reviews (2)

Cat no : CL555-11739

Synonyms

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

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Validation Data Gallery

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  IF Staining of rat liver using CL555-11739

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CoraLite®555 ASGR1 antibody (CL555-11739) at dilution of 1:200.

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  IF Staining of rat liver using CL555-11739

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CoraLite®555 ASGR1 antibody (CL555-11739) at dilution of 1:200.

• 

  IF Staining of human liver cancer using CL555-11739

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®555 ASGR1 antibody (CL555-11739) at dilution of 1:200.

• 

  IF Staining of human liver cancer using CL555-11739

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®555 ASGR1 antibody (CL555-11739) at dilution of 1:200.

• 

  FC experiment of HepG2 using CL555-11739

  1X10^6 HepG2 cells were surface stained with 0.4 ug CoraLite®555 Anti-Human ASGR1 (CL555-11739) (red), or 0.4 ug Control Antibody. Cells were not fixed .

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Tested Applications

• Positive IF-P detected in: rat liver tissue, human liver cancer tissue
• Positive FC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-11739 targets ASGR1 in IF-P, FC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASGR1 fusion protein Ag2310
• Full Name: asialoglycoprotein receptor 1
• Calculated Molecular Weight: 291 aa, 33 kDa
• GenBank Accession Number: BC032130
• Gene Symbol: ASGR1
• Gene ID (NCBI): 432
• RRID: AB_2919605
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rupa (Verified Customer) (04-26-2024)

Worked very well with immunofluorescence and flowcytometry

• Applications: Immunohistochemistry, Flow Cytometry
• Primary Antibody Dilution: 1-100
• Cell Tissue Type: mouse liver

FH

Parmita (Verified Customer) (04-23-2024)

worked fine for us. signal was sharp and specific detection observed

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse liver tissue

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