CoraLite® Plus 488-conjugated ASGR1 Polyclonal antibody

ASGR1 Polyclonal Antibody for FC, IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

Cat no : CL488-11739

Synonyms

ASGP R 1, ASGPR, ASGPR 1, ASGR1, asialoglycoprotein receptor 1, CLEC4H1, Hepatic lectin H1, HL 1, Hs.12056

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Validation Data Gallery

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  IF Staining of human liver cancer using CL488-11739

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 ASGR1 antibody (CL488-11739) at dilution of 1:200.

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  IF Staining of rat liver using CL488-11739

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CoraLite® Plus 488 ASGR1 antibody (CL488-11739) at dilution of 1:200.

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  FC experiment of HepG2 using CL488-11739

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human ASGR1 (CL488-11739) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were not fixed .

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Tested Applications

• Positive IF detected in: rat liver tissue, human liver cancer tissue
• Positive FC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL488-11739 targets ASGR1 in IF, FC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASGR1 fusion protein Ag2310
• Full Name: asialoglycoprotein receptor 1
• Calculated Molecular Weight: 291 aa, 33 kDa
• GenBank Accession Number: BC032130
• Gene Symbol: ASGR1
• Gene ID (NCBI): 432
• RRID: AB_2883080
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Publications

Species	Application	Title
mouse	IF	MedComm (2020); LILAR, a novel long noncoding RNA regulating autophagy in the liver tissues of endotoxemic mice through a competing endogenous RNA mechanism; Authors - Tian Tian