CoraLite®594-conjugated ASGR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ASGR1 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

Cat no : CL594-11739

Synonyms

ASGP R 1, ASGPR, ASGPR 1, Asgpr1, ASGR1, asialoglycoprotein receptor 1, CLEC4H1, Hepatic lectin H1, HL 1, Hs.12056

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Validation Data Gallery

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  IF Staining of rat liver using CL594-11739

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CoraLite®594 ASGR1 antibody (CL594-11739) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat liver using CL594-11739

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CoraLite®594 ASGR1 antibody (CL594-11739) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using CL594-11739

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 ASGR1 antibody (CL594-11739) at dilution of 1:200.

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  IF Staining of human liver cancer using CL594-11739

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 ASGR1 antibody (CL594-11739) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: rat liver tissue, human liver cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-11739 targets ASGR1 in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASGR1 fusion protein Ag2310
• Full Name: asialoglycoprotein receptor 1
• Calculated Molecular Weight: 291 aa, 33 kDa
• GenBank Accession Number: BC032130
• Gene Symbol: ASGR1
• Gene ID (NCBI): 432
• RRID: AB_2919773
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

Publications

Species	Application	Title
human	IF	iScience; ASGR1 is a promising target for lipid reduction in pigs with PON2 as its inhibitor; Authors - Yunjun Yin