ASGR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ASGR1 Polyclonal Antibody for WB, IHC, IF-P, FC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, FC, IP, CoIP, ELISA, Blocking assay

Conjugate

Unconjugated

Publications

20

Reviews (3)

Cat no : 11739-1-AP

Synonyms

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

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Validation Data Gallery

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  WB analysis using 11739-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11739-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11739-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11739-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 11739-1-AP

  IP result of anti-ASGR1 (IP:11739-1-AP, 4ug; Detection:11739-1-AP 1:2000) with mouse liver tissue lysate 4000 ug.

• 

  IHC staining of rat liver using 11739-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11739-1-AP (ASGR1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 11739-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11739-1-AP (ASGR1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11739-1-AP (ASGR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11739-1-AP (ASGR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 11739-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11739-1-AP (ASGR1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 11739-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11739-1-AP (ASGR1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver using 11739-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver tissue using ASGR1 antibody (11739-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA502, orange), CoraLite® Plus 488 CD163 antibody (CL488-16646, green), CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005, magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 11739-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ASGR1 antibody (11739-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human liver cancer using 11739-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ASGR1 antibody (11739-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat liver using 11739-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using ASGR1 antibody (11739-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 11739-1-AP

  1x10^6 HepG2 cells were surface stained with 0.4 ug ASGR1 Polyclonal antibody (11739-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were not fixed.

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Tested Applications

• Positive WB detected in: HepG2 cells, human liver tissue, mouse liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: rat liver tissue, human liver cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue, human liver tissue, rat liver tissue
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• FC: See 4 publications below
• CoIP: See 1 publications below

Product Information

11739-1-AP targets ASGR1 in WB, IHC, IF-P, FC, IP, CoIP, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASGR1 fusion protein Ag2310
• Full Name: asialoglycoprotein receptor 1
• Calculated Molecular Weight: 291 aa, 33 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC032130
• Gene Symbol: ASGR1
• Gene ID (NCBI): 432
• RRID: AB_2059675
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

Publications

Species	Application	Title
mouse	FC	Nature; Regulated control of gene therapies by drug-induced splicing.; Authors - Alex Mas Monteys
mouse	FC	Nano Lett; A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo.; Authors - Khalid A Hajj
human	IF	Acta Biomater; Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply.; Authors - Kun Du
human	WB	Biomaterials; Dual targeted delivery of statins and nucleic acids by chitosan-based nanoparticles for enhanced antiatherosclerotic efficacy.; Authors - Ting Jiang
human	WB	Cancer Res; ASO-Based PKM Splice-Switching Therapy Inhibits Hepatocellular Carcinoma Growth.; Authors - Wai Kit Ma
human	IHC,IF	Mol Ther; Enhanced Potency of GalNAc-Conjugated Antisense Oligonucleotides in Hepatocellular Cancer Models.; Authors - Youngsoo Kim

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

Mouse liver lysates were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:25000 incubated at 4 degree C overnight. ASGR1 bands appeared at about 42 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:25000
• Cell Tissue Type: Mouse liver tissue

FH

Kamal (Verified Customer) (02-15-2024)

Mouse liver lysates were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:25000 incubated at 4 degree C overnight. ASGR1 bands appeared at about 42 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:25000
• Cell Tissue Type: Mouse liver tissue

FH

James (Verified Customer) (11-18-2022)

Very good antibody! Used it to label hepatocytes in section of paraffin-embedded liver tissue

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Liver

ASGR1 Antibody Immunofluorescence validation (1/1000 dilution) in Liver (Cat no:11739-1-AP)

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