Beta Actin Monoclonal antibody, PBS Only

Beta Actin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat, plant, Zebrafish

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

7D2C10

Cat no : 60008-1-PBS

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin

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Validation Data Gallery

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  WB analysis of multi-cells/tissue using 60008-1-Ig (same clone as 60008-1-PBS)

  Western blot analysis of Beta-actin in various tissues and cell lines using Proteintech antibody 60008-1-Ig at a dilution of 1:5000. Extra bands were detected in some species with unknown reason. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa cells using 60008-1-Ig (same clone as 60008-1-PBS)

  Western blot of Hela cell with anti-Actin-Beta (60008-1-Ig) at various dilutions. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  WB analysis of arabidopsis whole plant using 60008-1-Ig (same clone as 60008-1-PBS)

  arabidopsis whole plant tissue were subjected to SDS PAGE followed by western blot with 60008-1-Ig (beta actin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60008-1-Ig (same clone as 60008-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60008-1-Ig (ACTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60008-1-Ig (same clone as 60008-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60008-1-Ig (ACTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 60008-1-Ig (same clone as 60008-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 60008-1-Ig (ACTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60008-1-Ig (same clone as 60008-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60008-1-Ig (ACTB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  WB analysis of rice whole plant using 60008-1-Ig (same clone as 60008-1-PBS)

  rice whole plant tissue were subjected to SDS PAGE followed by western blot with 60008-1-Ig (beta Actin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 60008-1-Ig(ACTB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 60008-1-Ig(ACTB antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 60008-1-Ig (beta actin Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 60008-1-Ig (beta actin Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60008-1-Ig (beta Actin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60008-1-Ig (beta Actin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 60008-1-Ig (beta actin Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60008-1-PBS targets Beta Actin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, plant, Zebrafish samples.

• Tested Reactivity: human, mouse, rat, plant, Zebrafish
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: Beta Actin fusion protein Ag0297
• Full Name: actin, beta
• Calculated Molecular Weight: 375 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC002409
• Gene Symbol: Beta Actin
• Gene ID (NCBI): 60
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.