Anticorps Polyclonal de lapin anti-ubiquitin

ubiquitin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (4)

Applications

WB, IHC, IF/ICC, FC (Intra), CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

421

Avis (6)

N° de cat : 10201-2-AP

Synonymes

ubiquitin B, UBB, Polyubiquitin-B, Polyubiquitin B

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Galerie de données de validation

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  WB analysis using 10201-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10201-2-AP (ubiquitin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10201-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10201-2-AP (ubiquitin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10201-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ubiquitin antibody (10201-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 10201-2-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ubiquitin (10201-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules MCF-7, cellules MDA-MB-231
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 336 publications below
• IHC: See 9 publications below
• IF: See 18 publications below
• CoIP: See 26 publications below
• ChIP: See 1 publications below

Informations sur le produit

10201-2-AP cible ubiquitin dans les applications de WB, IHC, IF/ICC, FC (Intra), CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, singe, souris, arabidopsis, Trypanosoma cruzi
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ubiquitin Protéine recombinante Ag0260
• Nom complet: ubiquitin B
• Numéro d’acquisition GenBank: BC000379
• Symbole du gène: ubiquitin
• Identification du gène (NCBI): 7314
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.

Publications

Species	Application	Title
mouse	WB	Nature; CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization; Authors - Kevin Qian
Trypanosoma cruzi	WB	Nature; Proteasome inhibition for treatment of leishmaniasis, Chagas disease and sleeping sickness.; Authors - Shilpi Khare
	WB	Cell Res; NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.; Authors - Min Liu
	WB	Nat Commun; UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean; Authors - Jiaqi Sun
human		Nat Commun; Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells; Authors - Qiuhong Zhu
human	IHC,IF	Acta Neuropathol; Upstream open reading frame with NOTCH2NLC GGC expansion generates polyglycine aggregates and disrupts nucleocytoplasmic transport: implications for polyglycine diseases.; Authors - Shaoping Zhong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

David (Verified Customer) (01-02-2024)

Various bands in the soluble fraction, potentially from conjugated proteins, but also clear bands for the ubiquitin monomers.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: SH-SY5Y lysate

FH

Brice-Emmanuel (Verified Customer) (10-12-2023)

Work well

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: Mouse Heart Tissue

ubiquitin Antibody Western Blot validation (1/500 dilution) in Mouse Heart Tissue (Cat no:10201-2-AP)

FH

Brandon (Verified Customer) (03-14-2022)

The antibody works perfectly for ubiquitin located in Human Fibroblast

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HFFs

ubiquitin Antibody Western Blot validation (1:1000 dilution) in HFFs (Cat no:10201-2-AP)

FH

Anita (Verified Customer) (08-06-2021)

10ug protein load from C. elegans

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C. elegans

ubiquitin Antibody Western Blot validation (1:1000 dilution) in C. elegans (Cat no:10201-2-AP)

FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using ubiquitin polyclonal antibody in NSC34 cell line at dilution of 1:500.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: NSC34

FH

Robert (Verified Customer) (06-24-2019)

1. reducing conditions2. non-reducing conditions

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: ST cells (porcine)

ubiquitin Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:10201-2-AP)

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