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NEDD4 Polyclonal antibody
NEDD4 Polyclonal Antibody for IHC, IP, WB, ELISA
Cat no : 21698-1-AP
Validation Data Gallery
|Positive WB detected in
|A549 cells, C6 cells, HeLa cells, HT-1080 cells, Jurkat cells, HEK-293 cells, NIH/3T3 cells, COS-7 cells
|Positive IP detected in
|Positive IHC detected in
|human breast cancer tissue, human lung cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:1000-1:8000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
21698-1-AP targets NEDD4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
|human, mouse, rat, monkey
|human, mouse, rat, chicken
|Host / Isotype
|Rabbit / IgG
|NEDD4 fusion protein Ag16291
|neural precursor cell expressed, developmentally down-regulated 4
|Calculated molecular weight
|1319 aa, 149 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 AND NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).
Proc Natl Acad Sci U S A
Zbtb7b engages the long noncoding RNA Blnc1 to drive brown and beige fat development and thermogenesis.
PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex.
Cell Death Differ
Ubiquitin ligase CHIP regulates OTUD3 stability and suppresses tumour metastasis in lung cancer.
Clin Transl Med
Tumor-derived exosomal miR-19b-3p facilitates M2 macrophage polarization and exosomal LINC00273 secretion to promote lung adenocarcinoma metastasis via Hippo pathway.
Xinbao Pill attenuated chronic heart failure by suppressing the ubiquitination of β-adrenergic receptors
Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity via Inhibiting USP33-Mediated ADRB2 Deubiquitination.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tongbin (Verified Customer) (08-25-2020)
This NEDD4 antibody works very well in western blots.