UBA52 Polyclonal antibody

UBA52 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 18039-1-AP

Synonyms

CEP52, HUBCEP52, RPL40, UBA52, UBCEP2

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Validation Data Gallery

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  IHC staining of mouse ovary using 18039-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 18039-1-AP (UBA52 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 18039-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 18039-1-AP (UBA52 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18039-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18039-1-AP (UBA52 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 18039-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18039-1-AP (UBA52 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 18039-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18039-1-AP (UBA52 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 18039-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18039-1-AP (UBA52 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 18039-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18039-1-AP (UBA52 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 18039-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18039-1-AP (UBA52 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: mouse ovary tissue, human kidney tissue, human placenta tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

18039-1-AP targets UBA52 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBA52 fusion protein Ag12669
• Full Name: ubiquitin A-52 residue ribosomal protein fusion product 1
• Calculated Molecular Weight: 128 aa, 15 kDa
• GenBank Accession Number: BC101830
• Gene Symbol: UBA52
• Gene ID (NCBI): 7311
• RRID: AB_2035029
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBA52 is a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.

Publications

Species	Application	Title
human	WB	Mediators Inflamm; UBC Mediated by SEPT6 Inhibited the Progression of Prostate Cancer.; Authors - Ruochen Zhang
human	WB	PLoS One; Ubiquitin B in cervical cancer: critical for the maintenance of cancer stem-like cell characters.; Authors - Yuan Tian