UBC12 Polyclonal antibody

• Featured Product
• KD/KO Validated

UBC12 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 14520-1-AP

Synonyms

UBE2M, NEDD8-conjugating enzyme Ubc12, NEDD8 protein ligase, NEDD8 conjugating enzyme Ubc12, NEDD8 carrier protein

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Validation Data Gallery

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  WB analysis of HEK-293 using 14520-1-AP

  WB result of UBC12 antibody (14520-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBC12 transfected HEK-293 cells.

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  WB analysis using 14520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14520-1-AP (UBC12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 14520-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14520-1-AP (UBC12 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of L02 using 14520-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14520-1-AP (UBC12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 14520-1-AP

  IP result of anti-UBC12 (IP:14520-1-AP, 4ug; Detection:14520-1-AP 1:600) with L02 cells lysate 1800ug.

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  IHC staining of mouse ovary using 14520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14520-1-AP (UBC12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 14520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14520-1-AP (UBC12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of L02 using 14520-1-AP

  Immunofluorescent analysis of L02 cells using 14520-1-AP (UBC12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, L02 cells, SMMC-7721 cells, HEK-293 cells, Ramos cells
• Positive IP detected in: L02 cells
• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

14520-1-AP targets UBC12 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBC12 fusion protein Ag5984
• Full Name: ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC058924
• Gene Symbol: UBC12
• Gene ID (NCBI): 9040
• RRID: AB_2878063
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation.; Authors - Haibin Zhou
human	WB	Elife; Genome-wide CRISPR screen identifies noncanonical NF-κB signaling as a regulator of density-dependent proliferation.; Authors - Maria Fomicheva ; KO Validated
human	WB	Cancer Biol Med; CUL4 E3 ligase regulates the proliferation and apoptosis of lung squamous cell carcinoma and small cell lung carcinoma.; Authors - Ting Li
human	IHC,WB, IF	Exp Cell Res; NPRL2 reduces the niraparib sensitivity of castration-resistant prostate cancer via interacting with UBE2M and enhancing neddylation.; Authors - Xin Zhao
human	WB	Biochem Biophys Res Commun; Disruption of protein neddylation with MLN4924 attenuates paclitaxel-induced apoptosis and microtubule polymerization in ovarian cancer cells.; Authors - Xiaoling Hong
human	WB	Biochem Cell Biol; Evaluation of HZX-960, a novel DCN1-UBC12 interaction inhibitor, as a potential antifibrotic compound for liver fibrosis.; Authors - Wenjuan Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Maria (Verified Customer) (02-06-2018)

I tested a free sample of this antibody.No non-specific bands.Load 40ug of protein for a well (15 well comb)I wish I could use a higher dilution of the antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:250-1:500
• Cell Tissue Type: EpH4 mammary gland cell line

UBC12 Antibody Western Blot, validation (1:250-1:500 dilution) in EpH4 mammary gland cell line (Cat no:14520-1-AP)