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  • KD/KO Validated

UBC12 Polyclonal antibody

UBC12 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14520-1-AP


hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12, NEDD8 protein ligase, UBC RS2, UBC12, UBE2M

Tested Applications

Positive WB detected inHEK-293T cells, L02 cells, SMMC-7721 cells, HEK-293 cells, Ramos cells
Positive IP detected inL02 cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14520-1-AP targets UBC12 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBC12 fusion protein Ag5984
Full Name ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC058924
Gene symbol UBE2M
Gene ID (NCBI) 9040
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.


Product Specific Protocols
WB protocol for UBC12 antibody 14520-1-APDownload protocol
IHC protocol for UBC12 antibody 14520-1-APDownload protocol
IF protocol for UBC12 antibody 14520-1-APDownload protocol
IP protocol for UBC12 antibody 14520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation.

Authors - Haibin Zhou


Genome-wide CRISPR screen identifies noncanonical NF-κB signaling as a regulator of density-dependent proliferation.

Authors - Maria Fomicheva
  • KO Validated

Cancer Biol Med

CUL4 E3 ligase regulates the proliferation and apoptosis of lung squamous cell carcinoma and small cell lung carcinoma.

Authors - Ting Li
humanIHC,WB, IF

Exp Cell Res

NPRL2 reduces the niraparib sensitivity of castration-resistant prostate cancer via interacting with UBE2M and enhancing neddylation.

Authors - Xin Zhao

Biochem Biophys Res Commun

Disruption of protein neddylation with MLN4924 attenuates paclitaxel-induced apoptosis and microtubule polymerization in ovarian cancer cells.

Authors - Xiaoling Hong

Biochem Cell Biol

Evaluation of HZX-960, a novel DCN1-UBC12 interaction inhibitor, as a potential antifibrotic compound for liver fibrosis.

Authors - Wenjuan Zhou


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Maria (Verified Customer) (02-06-2018)

I tested a free sample of this antibody.No non-specific bands.Load 40ug of protein for a well (15 well comb)I wish I could use a higher dilution of the antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:250-1:500
  • Cell Tissue Type: EpH4 mammary gland cell line
UBC12 Antibody Western Blot, validation (1:250-1:500 dilution) in EpH4 mammary gland cell line (Cat no:14520-1-AP)