CDC25A Polyclonal antibody

• Featured Product
• KD/KO Validated

CDC25A Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

31

Cat no : 55031-1-AP

Synonyms

CDC25A, CDC25A2, M phase inducer phosphatase 1

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Validation Data Gallery

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  WB analysis of HepG2 using 55031-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55031-1-AP (CDC25A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 55031-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55031-1-AP (CDC25A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 55031-1-AP

  IP result of anti-CDC25A (IP:55031-1-AP, 4ug; Detection:55031-1-AP 1:300) with HeLa cells lysate 4000ug.

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  IHC staining of human breast cancer using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human breast cancer using 55031-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 55031-1-AP (CDC25A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 30 publications below
• IHC: See 6 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

55031-1-AP targets CDC25A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cell division cycle 25 homolog A (S. pombe)
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_001789
• Gene Symbol: CDC25A
• Gene ID (NCBI): 993
• RRID: AB_2881258
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.

Publications

Species	Application	Title
human	WB	Nat Commun; The REGγ inhibitor NIP30 increases sensitivity to chemotherapy in p53-deficient tumor cells.; Authors - Xiao Gao
human	WB	Nat Commun; Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.; Authors - Jiliang Xia
human	WB	Cancer Res; YTHDF1 promotes gastric carcinogenesis by controlling translation of FZD7.; Authors - Jingnan Pi
human	WB,IHC,IF	Cell Death Differ; Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer.; Authors - Soumyadip Das
mouse	WB	Carbohydr Polym; Sulfate oligosaccharide of Gracilaria lemaneiformis modulates type 1 immunity by restraining T cell activation.; Authors - Qingmei Liu
human	WB	Oncogene; PTBP3 splicing factor promotes hepatocellular carcinoma by destroying the splicing balance of NEAT1 and pre-miR-612.; Authors - Xisheng Yang