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CDC25A Polyclonal antibody

CDC25A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55031-1-AP

Synonyms

CDC25A, CDC25A2, M phase inducer phosphatase 1



Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

55031-1-AP targets CDC25A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cell division cycle 25 homolog A (S. pombe)
Calculated molecular weight 59 kDa
Observed molecular weight 60 kDa
GenBank accession numberNM_001789
Gene symbol CDC25A
Gene ID (NCBI) 993
RRIDAB_2881258
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.

Protocols

Product Specific Protocols
WB protocol for CDC25A antibody 55031-1-APDownload protocol
IHC protocol for CDC25A antibody 55031-1-APDownload protocol
IF protocol for CDC25A antibody 55031-1-APDownload protocol
IP protocol for CDC25A antibody 55031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

The REGγ inhibitor NIP30 increases sensitivity to chemotherapy in p53-deficient tumor cells.

Authors - Xiao Gao
humanWB

Nat Commun

Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.

Authors - Jiliang Xia
humanWB

Cancer Res

YTHDF1 promotes gastric carcinogenesis by controlling translation of FZD7.

Authors - Jingnan Pi
humanWB,IHC,IF

Cell Death Differ

Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer.

Authors - Soumyadip Das
mouseWB

Carbohydr Polym

Sulfate oligosaccharide of Gracilaria lemaneiformis modulates type 1 immunity by restraining T cell activation.

Authors - Qingmei Liu
humanWB

Oncogene

PTBP3 splicing factor promotes hepatocellular carcinoma by destroying the splicing balance of NEAT1 and pre-miR-612.

Authors - Xisheng Yang