Validation Data Gallery
|Positive WB detected in
|HepG2 cells, mouse liver tissue
|Positive IP detected in
|Positive IHC detected in
|human breast cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|human breast cancer tissue
|Western Blot (WB)
|WB : 1:500-1:3000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
55031-1-AP targets CDC25A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Host / Isotype
|Rabbit / IgG
|cell division cycle 25 homolog A (S. pombe)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.
|Product Specific Protocols
|WB protocol for CDC25A antibody 55031-1-AP
|IHC protocol for CDC25A antibody 55031-1-AP
|IF protocol for CDC25A antibody 55031-1-AP
|IP protocol for CDC25A antibody 55031-1-AP
|Click here to view our Standard Protocols
The REGγ inhibitor NIP30 increases sensitivity to chemotherapy in p53-deficient tumor cells.
Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.
YTHDF1 promotes gastric carcinogenesis by controlling translation of FZD7.
Cell Death Differ
Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer.
Sulfate oligosaccharide of Gracilaria lemaneiformis modulates type 1 immunity by restraining T cell activation.
PTBP3 splicing factor promotes hepatocellular carcinoma by destroying the splicing balance of NEAT1 and pre-miR-612.