UBC9 Polyclonal antibody

UBC9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10070-1-AP

Synonyms

UBE2I, SUMO-conjugating enzyme UBC9, RING-type E3 SUMO transferase UBC9, P18, EC:2.3.2.-

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Validation Data Gallery

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  WB analysis using 10070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10070-1-AP (UBC9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10070-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10070-1-AP (UBC9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10070-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10070-1-AP (UBC9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10070-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10070-1-AP (UBC9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10070-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBC9 antibody (10070-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse testis tissue, rat testis tissue
• Positive IHC detected in: human lung cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IP: See 1 publications below

Product Information

10070-1-AP targets UBC9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBC9 fusion protein Ag0111
• Full Name: ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC051289
• Gene Symbol: UBC9
• Gene ID (NCBI): 7329
• RRID: AB_513699
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

Publications

Species	Application	Title
human	WB	Biochem Biophys Res Commun; Novel progerin-interactive partner proteins hnRNP E1, EGF, Mel 18, and UBC9 interact with lamin A/C.; Authors - Zhong Nanbert N
human	WB,IP	Biochem Biophys Res Commun; SUMOylation of optineurin is critical for inhibiting interferon β production; Authors - Xifeng Hu
	WB	J Virol; Multiple-Site SUMOylation of FMDV 3C Protease and Its Negative Role in Viral Replication; Authors - Xiangju Wu
human	WB	Cancer Gene Ther; METTL3-mediated RanGAP1 promotes colorectal cancer progression through the MAPK pathway by recruiting YTHDF1; Authors - Rui Yang
human	WB	Chem Res Toxicol; Natural Product Baohuoside I Impairs the Stability and Membrane Location of MRP2 Reciprocally Regulated by SUMOylation and Ubiquitination in Hepatocytes; Authors - Zhen Zhao
human,mouse	WB	Int J Biol Sci; SUMO E3 ligase MUL1 inhibits lymph node metastasis of bladder cancer by mediating mitochondrial HSPA9 translocation; Authors - Jilin Wu