Validation Data Gallery
|Positive WB detected in
|Jurkat cells, rat spleen tissue, K-562 cells, HeLa cells, NIH/3T3 cells, RAW264.7 cells, Raji cells
|Positive IHC detected in
|human lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IHC : 1:50-1:500
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
14899-1-AP targets PSMD4 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|PSMD4 fusion protein Ag6691
|proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. PSMD4 is a 50-kD subunit of the regulatory complex of the 26S proteasome. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.
|Product Specific Protocols
|WB protocol for PSMD4 antibody 14899-1-AP
|IHC protocol for PSMD4 antibody 14899-1-AP
|IF protocol for PSMD4 antibody 14899-1-AP
|FC protocol for PSMD4 antibody 14899-1-AP
|Click here to view our Standard Protocols
Loss of nuclear UBE3A causes electrophysiological and behavioral deficits in mice and is associated with Angelman syndrome.
J Cell Biol
The transition zone protein Rpgrip1l regulates proteasomal activity at the primary cilium.
The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.
The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.
J Cell Sci
S5a binds to death receptor-6 to induce THP-1 monocytes to differentiate through the activation of the NF-κB pathway.
Mol Cell Proteomics
Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7.