Anticorps Polyclonal de lapin anti-Timp-3

• Phare
• Validé par KD/KO

Timp-3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 10858-1-AP

Synonymes

HSMRK222, K222, K222TA2, Metalloproteinase inhibitor 3, Protein MIG 5, SFD, Timp 3, TIMP3, Timp-3

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Galerie de données de validation

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  WB analysis using 10858-1-AP

  Human placenta tissue lysates were subjected to SDS PAGE followed by western blot with 10858-1-AP (Timp-3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10858-1-AP (Timp-3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 10858-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10858-1-AP (Timp-3 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 10858-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10858-1-AP (Timp-3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 1 publications below
• IHC: See 8 publications below

Informations sur le produit

10858-1-AP cible Timp-3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Timp-3 Protéine recombinante Ag1253
• Nom complet: TIMP metallopeptidase inhibitor 3
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 20-30 kDa
• Numéro d’acquisition GenBank: BC014277
• Symbole du gène: TIMP3
• Identification du gène (NCBI): 7078
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Timp-3 (TIMP metallopeptidase inhibitor 3), also known as SFD. It is expected to be located in extracellular space, and the protein is enriched in the placenta tissue and fat tissue. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation, and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. TIMP-3 is expressed as an unglycosylated 24 kDa and glycosylated 29 kDa protein with inhibitory activity against interstitial collagenase, stromelysin-1, and gelatinases A and B (PMID:11827795).

Publications

Species	Application	Title
		Theranostics; miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury.; Authors - Jing Shi
human	IHC	Oxid Med Cell Longev; Trehalose Protects Keratinocytes against Ultraviolet B Radiation by Activating Autophagy via Regulating TIMP3 and ATG9A.; Authors - Li Li
human		Int Immunopharmacol; Sublytic C5b-9 induces TIMP3 expression by glomerular mesangial cells via TRAF6-dependent KLF5 K63-linked ubiquitination in rat Thy-1 nephritis; Authors - Shuai Ying
	IHC	Sci Rep; AMPK deficiency in chondrocytes accelerated the progression of instability-induced and ageing-associated osteoarthritis in adult mice.; Authors - Sheng Zhou
	IHC	Cell Prolif; Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT-3 signalling pathway.; Authors - Yunjiao Wang
		Biomolecules; Tau Protein Modulates Perineuronal Extracellular Matrix Expression in the TauP301L-acan Mouse Model.; Authors - Sophie Schmidt

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