TIMP1 Polyclonal antibody

• 

  WB analysis of mouse lung using 10753-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10753-1-AP (TIMP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10753-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10753-1-AP (TIMP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10753-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10753-1-AP (TIMP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10753-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10753-1-AP (TIMP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

TIMP1 Polyclonal Antibody for IF, IHC, ELISA

Cat No. 10753-1-AP

Publications(36)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IHC, IF, ELISA

Synonyms

CLGI, Collagenase inhibitor, EPA, HCI, Metalloproteinase inhibitor 1, TIMP, TIMP 1, TIMP1, TIMP-1

---

Tested Applications

• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 8 publications below
• IF: See 1 publications below

Product Information

10753-1-AP targets TIMP1 in IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIMP1 fusion protein Ag1146
• Full Name: TIMP metallopeptidase inhibitor 1
• Calculated Molecular Weight: 23 kDa
• GenBank Accession Number: BC007097
• Gene Symbol: TIMP1
• Gene ID (NCBI): 7076
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01033
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tissue Inhibitor of Metalloproteinases-1 (TIMP1) is a naturally occurring protein in the human organism regulating the activity of matrix metalloproteinases (MMPs). TIMP1 encodes a 931 base-pair mRNA and a 207 amino acid protein. Overexpression of TIMP1 can lead to a substantially increase of genes involved in proliferation, apoptosis and signal transduction. In addition, TIMP1 was found to decrease tumor cell sensitivity to multiple anticancer drugs by activation of downstream pathways and exhibited anti-apoptotic activity. Specifically, the TIMP1 could degrade cyclinB1 and activate the NF-kβ signaling pathway to protect breast cancer cells against chemotherapy-induced cell death.

Publications

Species	Application	Title
mouse		Int J Biol Macromol; Anti-metastatic effects of antrodan, the Antrodia cinnamomea mycelia glycoprotein, in lung carcinoma cells.; Authors - Kuan-Ning Fa
human	IHC	Br J Pharmacol; Dual Inhibition of Cannabinoid-1 Receptor and iNOS Attenuates Obesity-induced Chronic Kidney Disease.; Authors - Shiran Udi
mouse	IHC	Sci Rep; FOXO transcription factors protect against the diet-induced fatty liver disease.; Authors - Xiaoyan Pan
		Cancer Cytopathol; A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens.; Authors - Yi-Zhen Liu
mouse		Sci Rep; Quercetin prevents hepatic fibrosis by inhibiting hepatic stellate cell activation and reducing autophagy via the TGF-β1/Smads and PI3K/Akt pathways.; Authors - Liwei Wu
human		Mar Drugs; 11-epi-Sinulariolide Acetate Reduces Cell Migration and Invasion of Human Hepatocellular Carcinoma by Reducing the Activation of ERK1/2, p38MAPK and FAK/PI3K/AKT/mTOR Signaling Pathways.; Authors - Jen-Jie Lin