TIMP1 Polyclonal antibody
TIMP1 Polyclonal Antibody for IF, IHC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10753-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 28 publications below |
| IHC | See 8 publications below |
| IF | See 1 publications below |
Product Information
10753-1-AP targets TIMP1 in IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TIMP1 fusion protein Ag1146 |
| Full Name | TIMP metallopeptidase inhibitor 1 |
| Calculated molecular weight | 23 kDa |
| GenBank accession number | BC007097 |
| Gene symbol | TIMP1 |
| Gene ID (NCBI) | 7076 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tissue Inhibitor of Metalloproteinases-1 (TIMP1) is a naturally occurring protein in the human organism regulating the activity of matrix metalloproteinases (MMPs). TIMP1 encodes a 931 base-pair mRNA and a 207 amino acid protein. Overexpression of TIMP1 can lead to a substantially increase of genes involved in proliferation, apoptosis and signal transduction. In addition, TIMP1 was found to decrease tumor cell sensitivity to multiple anticancer drugs by activation of downstream pathways and exhibited anti-apoptotic activity. Specifically, the TIMP1 could degrade cyclinB1 and activate the NF-kβ signaling pathway to protect breast cancer cells against chemotherapy-induced cell death.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TIMP1 antibody 10753-1-AP | Download protocol |
| IF protocol for TIMP1 antibody 10753-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Br J Pharmacol Dual Inhibition of Cannabinoid-1 Receptor and iNOS Attenuates Obesity-induced Chronic Kidney Disease. | ||
Sci Rep Quercetin prevents hepatic fibrosis by inhibiting hepatic stellate cell activation and reducing autophagy via the TGF-β1/Smads and PI3K/Akt pathways. | ||
Mar Drugs 11-epi-Sinulariolide Acetate Reduces Cell Migration and Invasion of Human Hepatocellular Carcinoma by Reducing the Activation of ERK1/2, p38MAPK and FAK/PI3K/AKT/mTOR Signaling Pathways. | ||
Mediators Inflamm Protective Effect of Astaxanthin on Liver Fibrosis through Modulation of TGF-β1 Expression and Autophagy. | ||
Toxicol Appl Pharmacol In vivo inhibitory activity of andrographolide derivative ADN-9 against liver cancer and its mechanisms involved in inhibition of tumor angiogenesis. |
