Validation Data Gallery
|Positive WB detected in||HeLa cells, MCF-7 cells, MDA-MB-453s cells, mouse liver tissue, rat liver tissue|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18165-1-AP targets MMP13 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig, rabbit, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MMP13 fusion protein Ag12653|
|Full Name||matrix metallopeptidase 13|
|Calculated molecular weight||471 aa, 54 kDa|
|Observed molecular weight||65-70 kDa, 50-55 kDa, 40 kDa|
|GenBank accession number||BC074808|
|Gene ID (NCBI)||4322|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).
|Product Specific Protocols|
|WB protocol for MMP13 antibody 18165-1-AP||Download protocol|
|IHC protocol for MMP13 antibody 18165-1-AP||Download protocol|
|IF protocol for MMP13 antibody 18165-1-AP||Download protocol|
|IP protocol for MMP13 antibody 18165-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Effects of IL-1β on MMP-9 Expression in Cementoblast-Derived Cell Line and MMP-Mediated Degradation of Type I Collagen.
Int J Oncol
25-HC decreases the sensitivity of human gastric cancer cells to 5-fluorouracil and promotes cells invasion via the TLR2/NF-κB signaling pathway.
Cortistatin binds to TNF-α receptors and protects against osteoarthritis.
J Cell Physiol
Reduced PDGF-AA in subchondral bone leads to articular cartilage degeneration after strenuous running.
miR-93-5p attenuates IL-1β-induced chondrocyte apoptosis and cartilage degradation in osteoarthritis partially by targeting TCF4.
miR-100-5p-abundant exosomes derived from infrapatellar fat pad MSCs protect articular cartilage and ameliorate gait abnormalities via inhibition of mTOR in osteoarthritis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Xie (Verified Customer) (05-13-2021)
Good IHC staining for Rat MMP13.
Hongxue (Verified Customer) (01-12-2021)
clear wb band