DAPK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DAPK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 25136-1-AP

Synonyms

DAP kinase 1, DAPK, DAPK1

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Validation Data Gallery

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  WB analysis of A549 using 25136-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25136-1-AP (DAPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 25136-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25136-1-AP (DAPK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 25136-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25136-1-AP (DAPK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A549 using 25136-1-AP

  Immunofluorescent analysis of A549 cells using 25136-1-AP (DAPK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 25136-1-AP is DAPK1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DAPK1 fusion protein Ag18387
• Full Name: death-associated protein kinase 1
• Calculated Molecular Weight: 1430 aa, 160 kDa
• Observed Molecular Weight: 140-160 kDa
• GenBank Accession Number: BC113660
• Gene Symbol: DAPK1
• Gene ID (NCBI): 1612
• RRID: AB_2879917
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DAPK1(Death-associated protein kinase 1) is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. Loss of DAPK1 expression is associated with a selective advantage fortumor cells to resist apoptotic stimuli, allowing them to separate from the original tumor; from this point of view, DAPK1 could be considered a tumor metastases inhibitor gene(PMID:17319784). This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Life Sci; Auto- and paracrine rewiring of NIX-mediated mitophagy by insulin-like growth factor-binding protein 7 in septic AKI escalates inflammation-coupling tubular damage; Authors - Bang-Chuan Hu
human	WB, IF, IHC	Aging (Albany NY); Low DAPK1 expression correlates with poor prognosis and sunitinib resistance in clear cell renal cell carcinoma.; Authors - Zhengshuai Song
human	WB	Stem Cell Res Ther; Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.; Authors - Mengqi Chu ; KD Validated
mouse	WB	J Biol Chem; PMID: 28174303; Authors - Barbara Kroczynska
mouse	WB	J Alzheimers Dis; Oxytocin Alleviates MPTP-Induced Neurotoxicity in Mice by Targeting MicroRNA-26a/Death-Associated Protein Kinase 1 Pathway.; Authors - Hasan A M M Almansoub
human	WB,IHC	Stem Cells Dev; Reduced DAPK1 Expression Promotes Stem Cell-Like Characteristics of Prostate Cancer Cells by Activating ZEB1 via Hippo/YAP Signaling Pathway.; Authors - Shaojun Nong ; KD Validated