Anticorps Polyclonal de lapin anti-TRPC5

TRPC5 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 25890-1-AP

Synonymes

hTRP 5, hTRP5, Transient receptor protein 5, TRP 5, TRP5, TRPC5

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Galerie de données de validation

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  WB analysis using 25890-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25890-1-AP (TRPC5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 25890-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25890-1-AP (TRPC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 25890-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25890-1-AP (TRPC5 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 25890-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25890-1-AP (TRPC5 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cerebellum using 25890-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25890-1-AP (TRPC5 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cerebellum using 25890-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25890-1-AP (TRPC5 Antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of SH-SY5Y using 25890-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 25890-1-AP (TRPC5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules MCF-7
• Résultats positifs en IHC: tissu cérébral humain, tissu de cervelet humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IF: See 3 publications below

Informations sur le produit

25890-1-AP cible TRPC5 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRPC5 Protéine recombinante Ag23171
• Nom complet: transient receptor potential cation channel, subfamily C, member 5
• Masse moléculaire calculée: 111 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC137271
• Symbole du gène: TRPC5
• Identification du gène (NCBI): 7224
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Short transient receptor potential channel 5(TRPC5), which belongs to TRPC5 sub-subfamily of transient receptor (TC 1.A.4) family, thought to form a receptor-activated non-selective calcium permeant cation channel, and probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. TRPC5 has also been shown to be calcium-selective and may also be activated by intracellular calcium store depletion.

Publications

Species	Application	Title
human	WB	Cancer Lett; TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.; Authors - Da-Cen Lin
human	WB	Elife; Structural basis for human TRPC5 channel inhibition by two distinct inhibitors.; Authors - Kangcheng Song
mouse	WB,IF	J Neuroinflammation; Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.; Authors - Shiji Huo
human	WB	J Mol Cell Cardiol; TRPV6 protects ER stress-induced apoptosis via ATF6α-TRPV6-JNK pathway in human embryonic stem cell-derived cardiomyocytes.; Authors - Zhichao Li
mouse	IF	Biochem Pharmacol; Endothelial cell transient receptor potential channel C5 (TRPC5) is essential for endothelium-dependent contraction in mouse carotid arteries.; Authors - Cai Liang
human,mouse	WB	Biochem Biophys Res Commun; Trpc5-regulated AMPKα/mTOR autophagy pathway is associated with glucose metabolism disorders in low birth weight mice under overnutrition; Authors - Fei Zhou

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