TRPC4AP Monoclonal antibody

TRPC4AP Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C10B1

Cat no : 66138-1-Ig

Synonyms

C20orf188, Protein TAP1, Protein TRUSS, Trp4 associated protein, Trpc4 associated protein, TRPC4AP, TRRP4AP, TRUSS

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Validation Data Gallery

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  WB analysis of human brain using 66138-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66138-1-Ig (TRPC4AP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 66138-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66138-1-Ig (TRPC4AP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66138-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66138-1-Ig(TRPC4AP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 66138-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66138-1-Ig (TRPC4AP Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 66138-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66138-1-Ig (TRPC4AP Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 66138-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66138-1-Ig(TRPC4AP antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, human heart tissue
• Positive IHC detected in: human breast cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66138-1-Ig is TRPC4AP Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRPC4AP fusion protein Ag18772
• Full Name: transient receptor potential cation channel, subfamily C, member 4 associated protein
• Calculated Molecular Weight: 797 aa, 91 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC013144
• Gene Symbol: TRPC4AP
• Gene ID (NCBI): 26133
• RRID: AB_2881537
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPC4AP, also named as C20orf188 and TRRP4AP, is a substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. TRPC4AP participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. The calculated molecular weight of TRPC4AP is 91 kDa. Western blot analysis detected a protein of about 82 kDa expressed in several mouse cell lines and in a human promonocytic cell line.