TRPC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRPC1 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 19482-1-AP

Synonyms

HTRP 1, Transient receptor protein 1, TRP 1, TRP1, TRPC1

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Validation Data Gallery

• 

  WB analysis of human testis using 19482-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 19482-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 19482-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19482-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19482-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19482-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: PC-3 cells, human brain tissue, human testis tissue, mouse liver tissue, SH-SY5Y cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

19482-1-AP targets TRPC1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: transient receptor potential cation channel, subfamily C, member 1
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 90 kDa, 130 kDa
• GenBank Accession Number: NM_003304
• Gene Symbol: TRPC1
• Gene ID (NCBI): 7220
• RRID: AB_10642834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPC1, also named as TRP1, belongs to the transient receptor family and STrpC subfamily. TRPC1 is thought to form a receptor-activated non-selective calcium permeant cation channel. It has been reported that the mGluR1 evoked slow EPSC is mediated by the TRPC1 cation channel. It is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors.

Publications

Species	Application	Title
human	WB	Cell Res; Direct control of store-operated calcium channels by ultrafast laser.; Authors - Pan Cheng ; KD Validated
mouse	WB,IF	J Neuroinflammation; Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.; Authors - Shiji Huo
human	WB	J Biomed Sci; Chemoresistant ovarian cancer enhances its migration abilities by increasing store-operated Ca2+ entry-mediated turnover of focal adhesions.; Authors - Ho-Kai Huang
rat	WB	Sci Rep; Homer binds to Orai1 and TRPC channels in the neointima and regulates vascular smooth muscle cell migration and proliferation.; Authors - Shuping Jia
rat	WB	J Biochem Mol Toxicol; Galectin-3 mediates pulmonary vascular endothelial cell dynamics via TRPC1/4 under acute hypoxia.; Authors - Yumei Li
mouse	WB	Int J Mol Sci; STIM1 Knockout Enhances PDGF-Mediated Ca2+ Signaling through Upregulation of the PDGFR⁻PLCγ⁻STIM2 Cascade.; Authors - Tzu-Yu Huang

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