SOLO Polyclonal antibody

SOLO Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 23911-1-AP

Synonyms

SESTD1, SEC14 domain and spectrin repeat-containing protein 1, SEC14 and spectrin domains 1, Protein Solo, Huntingtin-interacting protein-like protein

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Validation Data Gallery

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  WB analysis using 23911-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23911-1-AP (SOLO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 23911-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23911-1-AP (SOLO antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 23911-1-AP

  IP result of anti-SOLO (IP:23911-1-AP, 4ug; Detection:23911-1-AP 1:4000) with mouse brain tissue lysate 1280 ug.

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  IP experiment of rat brain using 23911-1-AP

  IP result of anti-SOLO (IP:23911-1-AP, 0ug; Detection:23911-1-AP 1:4000) with rat brain tissue lysate 1120 ug.

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  IF Staining of HepG2 using 23911-1-AP

  Immunofluorescent analysis of HepG2 cells using 23911-1-AP (SOLO antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, MCF-7 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23911-1-AP targets SOLO in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOLO fusion protein Ag21016
• Full Name: SEC14 and spectrin domains 1
• Calculated Molecular Weight: 696 aa, 79 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC047578
• Gene Symbol: SOLO
• Gene ID (NCBI): 91404
• RRID: AB_2879360
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.