TRPC4AP Polyclonal antibody

TRPC4AP Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12606-1-AP

Synonyms

C20orf188, Protein TAP1, Protein TRUSS, Trp4 associated protein, Trpc4 associated protein, TRPC4AP, TRRP4AP, TRUSS

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Validation Data Gallery

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  WB analysis of human heart using 12606-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12606-1-AP (TRPC4AP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 12606-1-AP

  IP result of anti-TRPC4AP (IP:12606-1-AP, 5ug; Detection:12606-1-AP 1:200) with mouse heart tissue lysate 6000ug.

• 

  IHC staining of human breast cancer using 12606-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12606-1-AP (TRPC4AP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12606-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12606-1-AP (TRPC4AP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12606-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12606-1-AP (TRPC4AP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 12606-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 12606-1-AP (TRPC4AP Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 12606-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 12606-1-AP (TRPC4AP Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: human heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

12606-1-AP targets TRPC4AP in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRPC4AP fusion protein Ag3299
• Full Name: transient receptor potential cation channel, subfamily C, member 4 associated protein
• Calculated Molecular Weight: 797 aa, 91 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: BC013144
• Gene Symbol: TRPC4AP
• Gene ID (NCBI): 26133
• RRID: AB_2303776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPC4AP, also named as C20orf188 and TRRP4AP, is a substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. TRPC4AP participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. The calculated molecular weight of TRPC4AP is 91 kDa. Western blot analysis detected a protein of about 82 kDa expressed in several mouse cell lines and in a human promonocytic cell line.

Publications

Species	Application	Title
	WB	Genes Dev; Myc protein is stabilized by suppression of a novel E3 ligase complex in cancer cells.; Authors - Seung H Choi
human	WB	Oncogene; PP2A-B56α controls oncogene-induced senescence in normal and tumor human melanocytic cells.; Authors - Mannava S S
	WB	Cell Cycle; The G1 phase E3 ubiquitin ligase TRUSS that gets deregulated in human cancers is a novel substrate of the S-phase E3 ubiquitin ligase Skp2.; Authors - Azfar Jamal