|Positive WB detected in||A549 cells, mouse lung tissue|
|Positive IP detected in||A549 cells|
|Positive IHC detected in||human tonsillitis tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A549 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and N/A for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13750-1-AP targets MX1 in WB, IP, IHC, IF, FC applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||canine, Chicken, human, macaque, monkey, mouse, Pavo cristatus, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MX1 fusion protein Ag4692|
|Full Name||Myxovirus resistance protein 1|
|Calculated molecular weight||662 aa, 76 kDa|
|Observed molecular weight||76 kDa|
|GenBank accession number||BC032602|
|Gene ID (NCBI)||4599|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN. This antibody can recognize the endogenous Mx1 protein in several tissues like lung and spleen.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hannah (Verified Customer) (09-21-2020)
Used for western blot on human fibroblasts -/+ interferon treatment, detected single band only in IFN treated samples.