MYLK Polyclonal antibody

MYLK Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 21642-1-AP


Kinase related protein, KRP, MLCK, MLCK1, MLCK108, MLCK210, MSTP083, MYLK, MYLK1, myosin light chain kinase, smMLCK, Telokin

Tested Applications

Positive WB detected inmouse uterus tissue, mouse large intestine tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21642-1-AP targets MYLK in WB,IHC,IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYLK fusion protein Ag16306
Full Name myosin light chain kinase
Calculated Molecular Weight 1914 aa, 211 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberBC113456
Gene Symbol MYLK
Gene ID (NCBI) 4638
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYLK(Myosin light chain kinase) is also named as MLCK, MLCK1, MYLK1 and belongs to the protein kinase superfamily. It is a key enzyme in muscle contraction. Its activation is sufficient to enhance paracellular permeability and is required for tight junction barrier regulation in response to Na+-nutrient cotransport, inflammatory cytokines, or pathogenic bacteria(PMID:20404178). The expression of smooth muscle MYLK is tissue-specific. Smooth muscle MYLK generates three different proteins, long MYLK, short MYLK, and telokin. The 130-kDa smooth muscle, or short, MYLK (130 kDa) is expressed in smooth muscles. It has 8 isoforms produced by alternative splicing and alternative initiation and our anti-MYLK antibody can recognize these isforms.


Product Specific Protocols
WB protocol for MYLK antibody 21642-1-APDownload protocol
IHC protocol for MYLK antibody 21642-1-APDownload protocol
IF protocol for MYLK antibody 21642-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Research (Wash D C)

The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling

Authors - Xi Chen

Mucosal Immunol

Transmigrated neutrophils in the intestinal lumen engage ICAM-1 to regulate the epithelial barrier and neutrophil recruitment.

Authors - R Sumagin

Int J Biol Sci

Aryl Hydrocarbon Receptor Activation Modulates Intestinal Epithelial Barrier Function by Maintaining Tight Junction Integrity.

Authors - Min Yu


Dihydroartemisinin counteracts fibrotic portal hypertension via farnesoid X receptor-dependent inhibition of hepatic stellate cell contraction.

Authors - Wenxuan Xu

Front Med (Lausanne)

Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.

Authors - Fang He


Lanthanum chloride causes blood-brain barrier disruption through intracellular calcium-mediated RhoA/Rho kinase signaling and myosin light chain kinase.

Authors - Jie Wu