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TRPC4 Polyclonal antibody

TRPC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21349-1-AP

Synonyms

hTrp 4, HTRP4, Trp related protein 4, TRP4, TRPC4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TRPC4 Polyclonal antibody (21349-1-AP)

    WB analysis using 21349-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21349-1-AP (TRPC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TRPC4 Polyclonal antibody (21349-1-AP)

    IHC staining of human kidney using 21349-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21349-1-AP (TRPC4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TRPC4 Polyclonal antibody (21349-1-AP)

    IHC staining of human kidney using 21349-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21349-1-AP (TRPC4 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

21349-1-AP targets TRPC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPC4 fusion protein Ag15698
Full Name transient receptor potential cation channel, subfamily C, member 4
Calculated Molecular Weight 977 aa, 112 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC104725
Gene Symbol TRPC4
Gene ID (NCBI) 7223
RRIDAB_10734426
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TRPC4 antibody 21349-1-APDownload protocol
IHC protocol for TRPC4 antibody 21349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Sci Adv

Cryo-EM structure of TRPC5 at 2.8-Å resolution reveals unique and conserved structural elements essential for channel function.

Authors - Jingjing Duan
View Article
humanWB

Cancer Lett

TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.

Authors - Da-Cen Lin
View Article