Anticorps Polyclonal de lapin anti-Talin-1

• Phare
• Validé par KD/KO

Talin-1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(31)

Avis (2)

N° de cat : 14168-1-AP

Synonymes

ILWEQ, KIAA1027, talin 1, talin1, TLN

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Galerie de données de validation

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  WB analysis using 14168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14168-1-AP (Talin-1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14168-1-AP (Talin-1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14168-1-AP

  IP result of anti-Talin-1 (IP:14168-1-AP, 4ug; Detection:14168-1-AP 1:40000) with HeLa cells lysate 1320 ug.

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  IHC staining of human liver cancer using 14168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14168-1-AP (Talin-1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14168-1-AP (Talin-1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 14168-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14168-1-AP (Talin-1 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 14168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using Talin-1 antibody (14168-1-AP) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of A549 using 14168-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (14168-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  IF Staining of A549 using 14168-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (14168-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 14168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14168-1-AP (Talin-1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 14168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14168-1-AP (Talin-1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton was labelled in red with 66031-1-Ig (alpha tubulin).

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  IF Staining of HepG2 using 14168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14168-1-AP (Talin-1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HUVEC using 14168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using Talin-1 antibody (14168-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 14168-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Talin-1 Polyclonal antibody (14168-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules C6, cellules HeLa, cellules HepG2, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HUVEC, cellules A549, cellules HeLa, cellules HepG2
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 22 publications below
• IHC: See 4 publications below
• IF: See 14 publications below
• IP: See 1 publications below

Informations sur le produit

14168-1-AP cible Talin-1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Talin-1 Protéine recombinante Ag5367
• Nom complet: talin 1
• Masse moléculaire calculée: 2541 aa, 270 kDa
• Poids moléculaire observé: 250-270 kDa
• Numéro d’acquisition GenBank: BC042923
• Symbole du gène: Talin-1
• Identification du gène (NCBI): 7094
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

Publications

Species	Application	Title
human	IF	ACS Nano; Nanotopography Enhances Dynamic Remodeling of Tight Junction Proteins through Cytosolic Liquid Complexes.; Authors - Xiao Huang
human	WB	Antiviral Res; MβCD inhibits SFTSV entry by disrupting lipid raft structure of the host cells; Authors - Min Cheng
human	WB,IP,IHC,IF	Elife; Binding blockade between TLN1 and integrin β1 represses triple-negative breast cancer.; Authors - Yixiao Zhang ; KD Validated
human	IF	Mol Oncol; ITIH5 as a multifaceted player in pancreatic cancer suppression, impairing tyrosine kinase signaling, cell adhesion and migration; Authors - Jennifer Kosinski
chicken	WB	Microbiol Spectr; Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.; Authors - Beibei Zhang ; KD Validated
mouse	IF	Mol Neurobiol; Essential Role of Sptan1 in Cochlear Hair Cell Morphology and Function Via Focal Adhesion Signaling.; Authors - Qingxiu Yao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-12-2025)

Worked well for both WB and IF

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: WB 1:2000, IF, 1:300
• Cell Tissue Type: MSCs

FH

Elham (Verified Customer) (05-09-2025)

Really good staining. Used 1:200 dilution overnight at 4C and stained using Alexa Fluor 488 and actin Cell Mask 647

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human Airway Smooth Muscle Cell

Talin-1 Antibody Immunofluorescence validation (1:200 dilution) in Human Airway Smooth Muscle Cell (Cat no:14168-1-AP)

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