RIAM Polyclonal antibody

• Featured Product
• KD/KO Validated

RIAM Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13500-1-AP

Synonyms

APBB1 interacting protein 1, APBB1IP, INAG1, PREL 1, PREL1, Proline rich EVH1 ligand 1, Proline rich protein 73, RARP 1, RARP1, RIAM

---

Validation Data Gallery

• 

  WB analysis using 13500-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13500-1-AP (RIAM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 13500-1-AP

  IP result of anti-RIAM (IP:13500-1-AP, 5ug; Detection:13500-1-AP 1:500) with Jurkat cells lysate 2400ug.

• 

  IHC staining of human lymphoma using 13500-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13500-1-AP (RIAM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13500-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13500-1-AP (RIAM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

13500-1-AP targets RIAM in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RIAM fusion protein Ag4327
• Full Name: amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein
• Calculated Molecular Weight: 666 aa, 73 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC035636
• Gene Symbol: RIAM
• Gene ID (NCBI): 54518
• RRID: AB_2057231
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APBB1IP gene encodes a Rap1-GTP-interacting adapter molecule (RIAM) which is an important protein in Rap1-mediated integrin activation and adhesion. Rap1 is a small GTPase frequently activated in tumors such as melanoma and prostate cancer. RIAM is widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes. RIAM may function in the signal transduction from Ras activation to actin cytoskeletal remodeling. The observed molecular weight of RIAM is about 100 kDa.

Publications

Species	Application	Title
chicken	WB	Microbiol Spectr; Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.; Authors - Beibei Zhang ; KD Validated
mouse	WB	PLoS One; HPK1 associates with SKAP-HOM to negatively regulate Rap1-mediated B-lymphocyte adhesion.; Authors - Königsberger Sebastian S
		Immunol Lett; Non-cleavable talin rescues defect in the T-cell conjugation of T-cells deficient in the immune adaptor SKAP1.; Authors - Daina Lim
human	IHC	Oncol Lett; Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma.; Authors - Bingsheng Yang