Paxillin Polyclonal antibody

Paxillin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (2)

Cat no : 10029-1-Ig

Synonyms

PXN

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Validation Data Gallery

• 

  WB analysis of mouse brain using 10029-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10029-1-Ig (PXN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10029-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10029-1-Ig (Paxillin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10029-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10029-1-Ig (PXN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10029-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10029-1-Ig (PXN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10029-1-Ig

  IP result of anti-Paxillin (IP:10029-1-Ig, 3ug; Detection:10029-1-Ig 1:1000) with mouse brain tissue lysate 7500ug.

• 

  IHC staining of human liver cancer using 10029-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10029-1-Ig(PXN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 10029-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10029-1-Ig(PXN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 10029-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10029-1-Ig (Paxillin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 10029-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using Paxillin antibody (10029-1-Ig) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue).

• 

  IF Staining of HepG2 using 10029-1-Ig

  Immunofluorescent analysis of -20℃ Ethanol fixed HepG2 cells using 10029-1-Ig (Paxillin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton was labelled in red with 66031-1-Ig (alpha tubulin). DAPI (blue).

• 

  IF Staining of HepG2 using 10029-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10029-1-Ig (Paxillin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NIH/3T3 using 10029-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed NIH/3T3 cells using 10029-1-Ig(Paxillin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, COLO 320 cells, Jurkat cells, MCF-7 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HUVEC cells, NIH/3T3 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 9 publications below

Product Information

10029-1-Ig targets Paxillin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: paxillin
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: NM_002859
• Gene Symbol: Paxillin
• Gene ID (NCBI): 5829
• RRID: AB_513929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PXN (paxillin) is a 68 kDa scaffold protein that interacts with multiple structural and signaling proteins and regulates cell adhesion, migration, proliferation, and apoptosis. PXN is thought to play an important role in tumor migration, invasion, and metastasis (21045234). PXN has been identified as a direct substrate of protein tyrosine phosphatase receptor-type T (PTPRT), a potent tumor suppressor gene. Increased phospho-PXN at tyrosine residue 88 (Y88) has been found as a common feature of human colon cancers (20133777).

Publications

Species	Application	Title
human	WB	Mol Cell; Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine.; Authors - Daniel Arango
rat	IHC	Sci Adv; Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction; Authors - Yuzhi Sun
human	IF	Bone Res; Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma; Authors - Ming Luo
human	IF	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
human	WB	Cell Death Dis; ZC3H15 promotes glioblastoma progression through regulating EGFR stability.; Authors - Jianbing Hou
human	IF	Acta Pharmacol Sin; Reduced intracellular chloride concentration impairs angiogenesis by inhibiting oxidative stress-mediated VEGFR2 activation.; Authors - Kai Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Thomas (Verified Customer) (09-22-2022)

Works in WB (pulldown via PAK1) and IF (costained with F-Actin in cyan)

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:100 (IF), 1:500 (WB)
• Cell Tissue Type: Human Brain Microvascular endothelial cell line

Paxillin Antibody Western Blot,Immunofluorescence validation (1:100 (IF), 1:500 (WB) dilution) in Human Brain Microvascular endothelial cell line (Cat no:10029-1-Ig)

FH

Boyan (Verified Customer) (03-11-2019)

For WB, besides the expected band, it also recognised some other non-specific bands; for IF, it could label the cell cortex localisation, partially co-localised with Actin.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF

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