CD61 / Integrin beta 3 Polyclonal antibody

CD61 / Integrin beta 3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

47

Reviews (2)

Cat no : 18309-1-AP

Synonyms

CD61, GP3A, GPIIIa, Integrin beta 3, Integrin beta-3, ITGB3

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Validation Data Gallery

• 

  WB analysis using 18309-1-AP

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 18309-1-AP (CD61 / Integrin Beta 3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood platelets using 18309-1-AP

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 18309-1-AP (CD61 / Integrin Beta 3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 18309-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18309-1-AP (CD61 / Integrin Beta 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 18309-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 18309-1-AP (CD61 / Integrin Beta 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 18309-1-AP

  IP result of anti-CD61 / Integrin Beta 3 (IP:18309-1-AP, 4ug; Detection:18309-1-AP 1:300) with mouse spleen tissue lysate 5200 ug.

• 

  IP experiment of mouse spleen using 18309-1-AP

  IP result of anti-CD61 / Integrin Beta 3 (IP:18309-1-AP, 4ug; Detection:18309-1-AP 1:500) with mouse spleen tissue lysate 1920 ug.

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Tested Applications

• Positive WB detected in: Human peripheral blood platelets, mouse spleen tissue, rat spleen tissue
• Positive IP detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 37 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Product Information

18309-1-AP targets CD61 / Integrin beta 3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC127667
• Gene Symbol: Integrin beta 3
• Gene ID (NCBI): 3690
• RRID: AB_2128759
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.

Publications

Species	Application	Title
human	WB	Sci Adv; Platelet-derived porous nanomotor for thrombus therapy.; Authors - Mimi Wan
	WB	Nat Commun; Targeting Gα13-integrin interaction ameliorates systemic inflammation.; Authors - Ni Cheng
human,mouse	IF	Biomaterials; Construction and evaluation of novel αvβ3 integrin ligand-conjugated ultrasmall star polymer micelles targeted glomerular podocytes through GFB permeation.; Authors - Chengyuan Huang
rat	WB	Theranostics; A blood circulation-prolonging peptide anchored biomimetic phage-platelet hybrid nanoparticle system for prolonged blood circulation and optimized anti-bacterial performance.; Authors - Peipei Jin
		Thromb Res; Repurposing of rilpivirine for preventing platelet β3 integrin-dependent thrombosis by targeting c-Src active autophosphorylation; Authors - Kui Liu
mouse	IF,CoIP	NPJ Regen Med; hUC-MSCs-derived MFGE8 ameliorates locomotor dysfunction via inhibition of ITGB3/ NF-κB signaling in an NMO mouse model; Authors - Huiming Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sathyanarayani (Verified Customer) (04-05-2023)

I would highly recommend this primary antibody if you are looking for ITGB3. It produced really clear strong bands at 100kDa MW when used for western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse lysed blood

FH

Sathyanarayani (Verified Customer) (04-05-2023)

The ITGB3 antibody produced really nice clear bands on the western blot at 110Kda MW. I would highly recommend using this if you are measuring ITGB3.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse blood

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