Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, MDCK cells, NIH/3T3 cells, PC-12 cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | mouse colon tissue, human breast cancer tissue, human prostate cancer tissue, human skeletal muscle tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, A549 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:60000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 101 publications below |
| IF | See 38 publications below |
| IP | See 1 publications below |
Product Information
26520-1-AP targets Vinculin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| Tested Reactivity | human, mouse, rat, canine |
| Cited Reactivity | human, mouse, rat, pig, rabbit, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24942 Product name: Recombinant human Vinculin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 777-1066 aa of BC039174 Sequence: PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ Predict reactive species |
| Full Name | vinculin |
| Calculated Molecular Weight | 1133 aa, 124 kDa |
| Observed Molecular Weight | 124 kDa |
| GenBank Accession Number | BC039174 |
| Gene Symbol | Vinculin |
| Gene ID (NCBI) | 7414 |
| RRID | AB_2868558 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P18206 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Vinculin antibody 26520-1-AP | Download protocol |
| IHC protocol for Vinculin antibody 26520-1-AP | Download protocol |
| IF protocol for Vinculin antibody 26520-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Electron transport chain inhibition increases cellular dependence on purine transport and salvage | ||
Blood Epigenetic Regulation of Non-canonical Menin Targets Modulates Menin Inhibitor Response in Acute Myeloid Leukemia | ||
ACS Nano Polyethylenimine-Modified Mesoporous Silica Nanoparticles Induce a Survival Mechanism in Vascular Endothelial Cells via Microvesicle-Mediated Autophagosome Release | ||
Adv Sci (Weinh) Highly Effective and Noninvasive Near-Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Elham (Verified Customer) (06-18-2025) | Human airway smooth muscle cells fixed after 1 h of plating. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X 100. Blocked with 0.1% BSA and incubated with primary antibody overnight at 4C at 1:100 dilution. Following day cells were washed and incubated for 1 h with Alexa 594 at 1:400 dilution.
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FH Siavash (Verified Customer) (01-17-2025) | The antibody was used for immunofluorescence staining of 2D cultured cells fixed with 1%PFA and permeabilized with Triton X100. Cells were incubated overnight at +4 degrees celcius. The staining was visible in both epifluorescence microscope and confocal fluorescence microscope. Also, used for immunostaining of formalin-fixed paraffin-embedded (FFPE) skin of mouse. The antigen retrieval was citrate buffer. Primary antibody was incubated overnight at +4 degrees celcius.
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FH Sammy (Verified Customer) (12-04-2023) | Very good antibody for detection of vinculin by WB.
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FH Verdiana (Verified Customer) (11-03-2022) | Band at about 140kDa. Great antibody! Bands were visible just after 0.5sec of exposure! Clear bands.
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FH Bettina (Verified Customer) (08-03-2022) | Antibody worked well!
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FH Silvia (Verified Customer) (02-14-2022) | Good sharp clear band in western blot
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FH Bhuvanasundar (Verified Customer) (10-12-2021) | Immunofluorescence staining with this antibody worked very well.
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