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  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-1-Ig)

    WB analysis using 66305-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-1-Ig)

    WB analysis using 66305-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-1-Ig)

    WB analysis using 66305-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig heart tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    WB analysis of pig heart using 66305-1-Ig

    pig heart tissue were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    IHC staining of mouse heart using 66305-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66305-1-Ig (Vinculin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    IHC staining of mouse heart using 66305-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66305-1-Ig (Vinculin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    IHC staining of human breast cancer using 66305-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    IHC staining of human breast cancer using 66305-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    IHC staining of human prostate cancer using 66305-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    IHC staining of human prostate cancer using 66305-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Vinculin Monoclonal antibody (66305-1-Ig)

    IF Staining of HeLa using 66305-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66305-1-Ig(Vinculin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using Vinculin Monoclonal antibody (66305-1-Ig)

    FC experiment of HeLa using 66305-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Vinculin Monoclonal antibody (66305-1-Ig, Clone:2B5A7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

"Vinculin Antibodies" Comparison

View side-by-side comparison of Vinculin antibodies from other vendors to find the one that best suits your research needs.

Vinculin Monoclonal antibody

Vinculin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 66305-1-Ig
Clone No.2B5A7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

VCL, MVCL, MV, Metavinculin, CMD1W

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHepG2 cells, pig heart tissue, U-251 cells, LNCaP cells, U-87 MG cells, C6 cells, HEK-293 cells, HeLa cells, HuH-7 cells, L02 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66305-1-Ig targets Vinculin in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, pig, rabbit, zebrafish, hamster
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag24946

Product name: Recombinant human Vinculin protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 777-1066 aa of BC039174

Sequence: PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Predict reactive species
Full Name vinculin
Calculated Molecular Weight 1133 aa, 124 kDa
Observed Molecular Weight 117 kDa
GenBank Accession NumberBC039174
Gene Symbol Vinculin
Gene ID (NCBI) 7414
RRIDAB_2810300
Conjugate Unconjugated
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP18206
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Protocols

Product Specific Protocols
WB protocol for Vinculin antibody 66305-1-IgDownload protocol
IHC protocol for Vinculin antibody 66305-1-IgDownload protocol
IF protocol for Vinculin antibody 66305-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Crit Care

Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.

Authors - Lingbing Zhang
humanWB

Adv Sci (Weinh)

RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation

Authors - Yu Sun
humanWB

Cell Rep Med

Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition

Authors - Yu Chang
humanWB

Nat Commun

Modular assembly of the principal microtubule nucleator γ-TuRC.

Authors - Martin Würtz
humanWB

Dev Cell

HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis

Authors - Xiang Peng
mouseIF

Circ. Res.

Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.

Authors - Dong Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Aditya (Verified Customer) (01-31-2025)

very clean bands, much better than the abcam antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
FH

Morgane (Verified Customer) (01-09-2025)

Very good loading control with larger molecular size

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HCT116
Vinculin Antibody Western Blot validation (1:1000 dilution) in HCT116 (Cat no:66305-1-Ig)
FH

Daniel (Verified Customer) (10-24-2024)

The antibody works really well and it gives a very clean Western blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: eHAP, RPE1, HCT116 cells
FH

Lisa (Verified Customer) (04-29-2024)

Works super well!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
FH

Parijat (Verified Customer) (08-21-2023)

Works well as loading control

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293T
FH

Udesh (Verified Customer) (08-16-2023)

Worked well in WB at 1:3000 and IF at 1:100

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Bone Marrow MSCs
FH

Mohamad (Verified Customer) (07-03-2023)

Very good antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
FH

Priya (Verified Customer) (01-17-2023)

I have used for human cardiomyocytes, mouse skin and liver tissues

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cardiomyoctyes, Keratinocytes, Liver and skin
FH

Macarena (Verified Customer) (10-07-2022)

excellent results.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: cell lines
FH

Jonas (Verified Customer) (07-29-2022)

This is my go to loading control stain to validate evenly loaded lanes Binding could be stronger but by using 1:500 dilution, a reliable staining can be achieved

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
Vinculin Antibody Western Blot validation (1:500 dilution) in (Cat no:66305-1-Ig)
FH

Charlotte (Verified Customer) (07-26-2022)

Very good antibody. Very specific, super fast to reveal. Here we see Gli1 (160 kDa) because it is a mouse antibody too.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/5000
  • Cell Tissue Type: NIH-3T3
Vinculin Antibody Western Blot validation (1/5000 dilution) in NIH-3T3 (Cat no:66305-1-Ig)
FH

AKIMASA (Verified Customer) (04-28-2021)

I could get the good quality band!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: OVCAR8, PEO4, Kuramochi, OVSAHO, A2780, HEY,PEO1
FH

Chun (Verified Customer) (09-07-2020)

This is a fairly good antibody for immunoblotting.

  • Applications: Western Blot
  • Cell Tissue Type: HeLa
FH

Huai-Chin (Verified Customer) (06-08-2019)

Serving as a loading control, this antibody is not that good compare to other. Still work to some extent.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: 293T
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