Anticorps Polyclonal de lapin anti-SIX2

• Phare
• Validé par KD/KO

SIX2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (6)

Applications

WB, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

249

Avis (1)

N° de cat : 11562-1-AP

Synonymes

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2

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Galerie de données de validation

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  WB analysis using 11562-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11562-1-AP (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11562-1-AP

  IP result of anti-SIX2 (IP:11562-1-AP, 4ug; Detection:11562-1-AP 1:1000) with HEK-293 cells lysate 1320 ug.

• 

  IF Staining of heritable frontonasal dysplasia and renal hypoplas using 11562-1-AP

  IF result from Fogelgren B, PMID:18570229, "Misexpression of Six2 is associated with heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice.".

• 

  IF Staining of mouse embryonic kidney using 11562-1-AP

  IF result of SIX2 antibody (11562-1-AP, 1:200) with mouse embryonic kidney rudiment dissected at E13.5 and cultured for 2 days by Dr. Aleksandra Rak-Raszewska. SIX2 positive cells (red) incondense metanephric mesenchyme surrounding the ureteric bud tip.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules L02, cellules PC-3
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IF: mouse embryonic kidney, heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 30 publications below
• IP: See 3 publications below
• ChIP: See 5 publications below

Informations sur le produit

11562-1-AP cible SIX2 dans les applications de WB, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, canin, Humain, Lapin, porc, souris, Alligator
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIX2 Protéine recombinante Ag2124
• Nom complet: SIX homeobox 2
• Masse moléculaire calculée: 291 aa, 32 kDa
• Poids moléculaire observé: 32-37 kDa
• Numéro d’acquisition GenBank: BC024033
• Symbole du gène: SIX2
• Identification du gène (NCBI): 10736
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599]. This antibody is a rabbit polyclonal antibody raised against full length SIX2 of human origin.

Publications

Species	Application	Title
human		Nature; Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.; Authors - Minoru Takasato
human		Nat Biotechnol; Tubuloids derived from human adult kidney and urine for personalized disease modeling.; Authors - Frans Schutgens
human		Nat Biotechnol; Revealing the spatio-phenotypic patterning of cells in healthy and tumor tissues with mLSR-3D and STAPL-3D.; Authors - Ravian L van Ineveld
mouse		Cell; In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation.; Authors - Hsin-Kai Liao
human		Cell; Tridimensional Visualization and Analysis of Early Human Development.; Authors - Morgane Belle
mouse		Cell Stem Cell; Redefining the in vivo origin of metanephric nephron progenitors enables generation of complex kidney structures from pluripotent stem cells.; Authors - Atsuhiro Taguchi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nicolas (Verified Customer) (09-17-2020)

I performed an antigen retrieval (boil in citrate buffer 10mM, pH6 during 12 minutes)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: E18.5 kidney

SIX2 Antibody Immunofluorescence validation (1/200 dilution) in E18.5 kidney (Cat no:11562-1-AP)

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