Validation Data Gallery
|Positive WB detected in||A2780 cells, HEK-293 cells, HepG2 cells, L02 cells, PC-3 cells, SKOV-3 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IF detected in||epithelial tumor cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10709-1-AP targets SIX1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SIX1 fusion protein Ag1044|
|Full Name||SIX homeobox 1|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||32-35 kDa|
|GenBank accession number||BC008874|
|Gene ID (NCBI)||6495|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.
Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.
Muscle progenitor specification and myogenic differentiation are associated with changes in chromatin topology.
Dynamic enhancers control skeletal muscle identity and reprogramming.
O-GlcNAcylation of SIX1 enhances its stability and promotes Hepatocellular Carcinoma Proliferation.
The homeoprotein SIX1 controls cellular senescence through the regulation of p16INK4A and differentiation-related genes.
Mol Ther Oncolytics
miR-489-3p/SIX1 Axis Regulates Melanoma Proliferation and Glycolytic Potential.