MYCN Polyclonal antibody

• Featured Product
• KD/KO Validated

MYCN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (4)

Cat no : 10159-2-AP

Synonyms

bHLHe37, MODED, MYCN, N myc, N myc proto oncogene protein, ODED

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Validation Data Gallery

• 

  WB analysis of human placenta using 10159-2-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 10159-2-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10159-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 10159-2-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10159-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10159-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10159-2-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human thyroid cancer using 10159-2-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10159-2-AP (MYCN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10159-2-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10159-2-AP (MYCN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue, SKOV-3 cells, C6 cells, mouse brain tissue, SH-SY5Y cells, rat brain tissue
• Positive IHC detected in: human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 10 publications below
• IF: See 1 publications below

Product Information

10159-2-AP targets MYCN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYCN fusion protein Ag0193
• Full Name: v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC002712
• Gene Symbol: MYCN
• Gene ID (NCBI): 4613
• RRID: AB_2266881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYCN, also named as BHLHE37 and NMYC, is a transcription factor. It is primarily expressed in normal developing embryos and is thought to be critical in brain and other neural development. It is often amplified in human neuroblastomas. IR34A can suppress MYCN expression, it suggest MYCN has a role in cell growth. The expression of the MCYN is upregulated in a variety of human tumors, most frequently neuroblastoma, where the level of expression appears to increase as the tumor progresses. The calculated molecuar weight of MYCN is 50 kDa, but Phosphorylated MYCN is about 65-70 kDa. MYCN exsits two isoforms and MV of isoform is respectively 65 kDa and 45 kDa. (PMID: 19615087 )

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB	Elife; Combined ALK and MDM2 inhibition increases antitumor activity and overcomes resistance in human ALK mutant neuroblastoma cell lines and xenograft models.; Authors - Hui Qin Wang ; KD Validated
human	WB,IHC	Cancers (Basel); Transcriptomic Analyses of MYCN-Regulated Genes in Anaplastic Wilms' Tumour Cell Lines Reveals Oncogenic Pathways and Potential Therapeutic Vulnerabilities.; Authors - Marianna Szemes ; KD Validated
mouse	IHC	Front Oncol; Downregulation of MYCN through PI3K Inhibition in Mouse Models of Pediatric Neural Cancer.; Authors - Tene Aneka Cage
human	IHC	Cancer; Neuroblastoma of undifferentiated subtype, prognostic significance of prominent nucleolar formation, and MYC/MYCN protein expression: a report from the Children's Oncology Group.; Authors - Larry L Wang
mouse	WB,IHC	Front Cell Dev Biol; An E2F5-TFDP1-BRG1 Complex Mediates Transcriptional Activation of MYCN in Hepatocytes.; Authors - Zhiwen Fan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

CHAO (Verified Customer) (03-31-2022)

Strong bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

FH

Tom (Verified Customer) (08-22-2020)

I saw a specific ~60 kDa band when performing western blots using this MYCN antibody. The band is bigger than molecular weight of MYCN, probably due to post-translational modifications.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart

FH

Tom (Verified Customer) (08-22-2020)

I performed immunofluorescence using this antibody. I observed very strong MYCN signal in mouse heart tissue sections. However, I saw cytoplasmic staining in addition to nuclear staining. It is probably due to MYCN's localization to both cytoplasm and nuclei or this antibody has some background staining.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse heart

FH

Jessica (Verified Customer) (10-31-2018)

Strongest band observed was around 60 kDa with some much weaker bands much higher and much lower observable with higher exposures. Ab had affinity for BE2C cell lysate, but did not bind the c-myc expressed in Sy5Y cells on the same blot. I used 2 % skim milk PBST for incubation. As MYCN Abs go this one is pretty good!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Neuroblastoma cell lines

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