|Positive WB detected in||MCF-7 cells, HEK-293 cells, HepG2 cells|
|Positive IP detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
55365-1-AP targets FOXC1 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||forkhead box C1|
|Calculated molecular weight||57 kDa|
|Observed molecular weight||70 kDa|
|GenBank accession number||NM_001453|
|Gene ID (NCBI)||2296|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
FOXC1, also named as FKHL7 and FREAC3, binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Defects in FOXC1 are the cause of Axenfeld-Rieger syndrome type 3 (RIEG3). Defects in FOXC1 are the cause of iridogoniodysgenesis anomaly (IGDA). Defects in FOXC1 are a cause of Peters anomaly. This antibody is specific to FOXC1. Phosphorylation modification of Foxc1 protein may be responsible for the larger molecular weight of detection compared with theoretical molecular weight (PMID:27708239;16403239).
The osteoporosis risk variant rs9820407 at 3p22.1 acts as an allele-specific enhancer to regulate CTNNB1 expression by long-range chromatin loop formation.
The long noncoding RNA FOXCUT promotes proliferation and migration by targeting FOXC1 in nasopharyngeal carcinoma.
Mol Med Rep
FOXC1 silencing inhibits the epithelial‑to‑mesenchymal transition of glioma cells: Involvement of β‑catenin signaling.
Icaritin inhibits endometrial carcinoma cells by suppressing O-GlcNAcylation of FOXC1
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sarah (Verified Customer) (08-20-2021)
I was sent 4 antibodies to try. You can see that JUN (1:1000) and NFKB1 (1:1000) worked, but FOXC1 (1:300) and HES1 (1:500) did not. I have shown that the cells express the mRNA for these proteins by qPCR.