SIX4 Polyclonal antibody

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  WB analysis using 21305-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21305-1-AP (SIX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21305-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21305-1-AP (SIX4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse salivary gland using 21305-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse salivary gland tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat salivary gland using 21305-1-AP

  Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 21305-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 21305-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 21305-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse tongue using 21305-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 21305-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21305-1-AP (SIX4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SIX4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21305-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SIX homeobox 4, Homeobox protein SIX4, AREC3

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells
• Positive IHC detected in: human cervical cancer tissue, human ovary cancer tissue, mouse salivary gland tissue, mouse tongue tissue, rat salivary gland tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

21305-1-AP targets SIX4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIX4 fusion protein Ag15918
• Full Name: SIX homeobox 4
• Calculated Molecular Weight: 781 aa, 83 kDa
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: BC098282
• Gene Symbol: SIX4
• Gene ID (NCBI): 51804
• RRID: AB_10860258
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UIU6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Six (sine oculis) proteins are a family of homeodomain transcription factors that share a conserved DNA-binding domain and are human homologs of the Drosophila sine oculis (so) protein. SIX homeobox 4 (SIX4), also known as AREC3, belongs to the the Six family. Six4 contains one Six domain (which funtions as a homeobox DNA-binding motif) and shares 90% sequence similarity with its mouse counterpart, suggesting that both proteins have similar DNA-binding properties. SIX4 is thought to be involved in neurogenesis, myogenesis, and development of other organs, based on its specific expression in certain neuronal cells of the developing embryo and in adult skeletal muscles.

Publications

Species	Application	Title
human	WB	EMBO Rep; LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1α-FOXO4-p21-dependent signaling axis.; Authors - Xuedan Sun
mouse	WB	iScience; RBFOX2 regulated EYA3 isoforms partner with SIX4 or ZBTB1 to control transcription during myogenesis; Authors - Hannah J Wiedner
mouse	IF	Dev Biol; A cellular and molecular mosaic establishes growth and differentiation states for cranial sensory neurons.; Authors - Beverly A Karpinski
human,mouse	WB,IHC,IF	Int J Biol Sci; SIX4 Activation in Inflammatory Response Drives the Transformation of Colorectal Epithelium into Inflammation and Tumor via Feedback-Enhancing Inflammatory Signaling to Induce Tumor Stemness Signaling; Authors - Ziyan Zhang ; KD Validated