SIX4 Polyclonal antibody

SIX4 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 21305-1-AP


AREC3, Homeobox protein SIX4, Sine oculis homeobox homolog 4, SIX homeobox 4, SIX4

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21305-1-AP targets SIX4 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIX4 fusion protein Ag15918
Full Name SIX homeobox 4
Calculated Molecular Weight 781 aa, 83 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC098282
Gene Symbol SIX4
Gene ID (NCBI) 51804
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Six (sine oculis) proteins are a family of homeodomain transcription factors that share a conserved DNA-binding domain and are human homologs of the Drosophila sine oculis (so) protein. SIX homeobox 4 (SIX4), also known as AREC3, belongs to the the Six family. Six4 contains one Six domain (which funtions as a homeobox DNA-binding motif) and shares 90% sequence similarity with its mouse counterpart, suggesting that both proteins have similar DNA-binding properties. SIX4 is thought to be involved in neurogenesis, myogenesis, and development of other organs, based on its specific expression in certain neuronal cells of the developing embryo and in adult skeletal muscles.


Product Specific Protocols
WB protocol for SIX4 antibody 21305-1-APDownload protocol
IF protocol for SIX4 antibody 21305-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1α-FOXO4-p21-dependent signaling axis.

Authors - Xuedan Sun


RBFOX2 regulated EYA3 isoforms partner with SIX4 or ZBTB1 to control transcription during myogenesis

Authors - Hannah J Wiedner

Dev Biol

A cellular and molecular mosaic establishes growth and differentiation states for cranial sensory neurons.

Authors - Beverly A Karpinski