EYA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

EYA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 22658-1-AP

Synonyms

BOP, BOR, EYA1, Eyes absent homolog 1

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Validation Data Gallery

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  WB analysis using 22658-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22658-1-AP (EYA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 22658-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 22658-1-AP (EYA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 22658-1-AP

  IP result of anti-EYA1 (IP:22658-1-AP, 4ug; Detection:22658-1-AP 1:4000) with HepG2 cells lysate 2600 ug.

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  IHC staining of human kidney using 22658-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22658-1-AP (EYA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 22658-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22658-1-AP (EYA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 22658-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EYA1 antibody (22658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse colon tissue, HuH-7 cells, NCCIT cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

22658-1-AP targets EYA1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EYA1 fusion protein Ag18489
• Full Name: eyes absent homolog 1 (Drosophila)
• Calculated Molecular Weight: 592 aa, 65 kDa
• Observed Molecular Weight: 61 kDa and 65 kDa
• GenBank Accession Number: BC121799
• Gene Symbol: EYA1
• Gene ID (NCBI): 2138
• RRID: AB_2879145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The EYA1 (Eyes absent homolog 1) gene is the human homologue of the Drosophila Eya1 gene, which is essential for eye development in that species. There are 4 different isoforms of EYA1 (EYA1A, EYA1B, EYA1C, EYA1D), which are composed of 559 amino acids (AA), 592 AA, 592 AA and 557 AA, respectively. It should be noted that isoforms B and C have the same length; however, they could be considered different variants because of change in amino acidic composition (PMID: 24803398).

Publications

Species	Application	Title
human	WB	Cell Rep; YTHDF1 phase separation triggers the fate transition of spermatogonial stem cells by activating the IκB-NF-κB-CCND1 axis; Authors - Qian Fang
human	IHC,IF	Front Oncol; SIX1 Activates STAT3 Signaling to Promote the Proliferation of Thyroid Carcinoma via EYA1.; Authors - Deguang Kong
human	WB,IHC	Oncotarget; Eyes absent gene (EYA1) is a pathogenic driver and a therapeutic target for melanoma.; Authors - Joshua Jiawei Zhou ; KD Validated
human	WB	Stem Cell Res Ther; Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model.; Authors - Qiuxia Cui
human	IHC	Clin Breast Cancer; Dachshund 1 is Differentially Expressed Between Male and Female Breast Cancer: A Matched Case-Control Study of Clinical Characteristics and Prognosis.; Authors - Qiuxia Cui
rat	WB,IP	J Mol Neurosci; MES23.5 DA Immortalized Neuroblastoma Cells Self-protect Against Early Injury by Overexpressing Glial Cell-derived Neurotrophic Factor via Akt1/Eya1/Six2 Signaling.; Authors - Jin Gao