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  • KD/KO Validated

EYA1 Polyclonal antibody

EYA1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 22658-1-AP


BOP, BOR, EYA1, Eyes absent homolog 1

Tested Applications

Positive WB detected inHepG2 cells, mouse colon tissue, HuH-7 cells, NCCIT cells, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22658-1-AP targets EYA1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EYA1 fusion protein Ag18489
Full Name eyes absent homolog 1 (Drosophila)
Calculated Molecular Weight 592 aa, 65 kDa
Observed Molecular Weight 61 kDa and 65 kDa
GenBank Accession NumberBC121799
Gene Symbol EYA1
Gene ID (NCBI) 2138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The EYA1 (Eyes absent homolog 1) gene is the human homologue of the Drosophila Eya1 gene, which is essential for eye development in that species. There are 4 different isoforms of EYA1 (EYA1A, EYA1B, EYA1C, EYA1D), which are composed of 559 amino acids (AA), 592 AA, 592 AA and 557 AA, respectively. It should be noted that isoforms B and C have the same length; however, they could be considered different variants because of change in amino acidic composition (PMID: 24803398).


Product Specific Protocols
WB protocol for EYA1 antibody 22658-1-APDownload protocol
IHC protocol for EYA1 antibody 22658-1-APDownload protocol
IF protocol for EYA1 antibody 22658-1-APDownload protocol
IP protocol for EYA1 antibody 22658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Rep

YTHDF1 phase separation triggers the fate transition of spermatogonial stem cells by activating the IκB-NF-κB-CCND1 axis

Authors - Qian Fang

Front Oncol

SIX1 Activates STAT3 Signaling to Promote the Proliferation of Thyroid Carcinoma via EYA1.

Authors - Deguang Kong


Eyes absent gene (EYA1) is a pathogenic driver and a therapeutic target for melanoma.

Authors - Joshua Jiawei Zhou
  • KD Validated

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Clin Breast Cancer

Dachshund 1 is Differentially Expressed Between Male and Female Breast Cancer: A Matched Case-Control Study of Clinical Characteristics and Prognosis.

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J Mol Neurosci

MES23.5 DA Immortalized Neuroblastoma Cells Self-protect Against Early Injury by Overexpressing Glial Cell-derived Neurotrophic Factor via Akt1/Eya1/Six2 Signaling.

Authors - Jin Gao