Validation Data Gallery
|Positive WB detected in||HepG2 cells, mouse kidney tissue, mouse colon tissue, HeLa cells, HuH-7 cells, NCCIT cells, mouse liver tissue, rat liver tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
22658-1-AP targets EYA1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EYA1 fusion protein Ag18489|
|Full Name||eyes absent homolog 1 (Drosophila)|
|Calculated molecular weight||592 aa, 65 kDa|
|Observed molecular weight||60 kDa, 50 kDa|
|GenBank accession number||BC121799|
|Gene ID (NCBI)||2138|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The EYA1 (Eyes absent homolog 1) gene is the human homologue of the Drosophila Eya1 gene, which is essential for eye development in that species. There are 4 different isoforms of EYA1 (EYA1A, EYA1B, EYA1C, EYA1D), which are composed of 559 amino acids (AA), 592 AA, 592 AA and 557 AA, respectively. It should be noted that isoforms B and C have the same length; however, they could be considered different variants because of change in amino acidic composition (PMID: 24803398).
|Product Specific Protocols|
|WB protocol for EYA1 antibody 22658-1-AP||Download protocol|
|IHC protocol for EYA1 antibody 22658-1-AP||Download protocol|
|IF protocol for EYA1 antibody 22658-1-AP||Download protocol|
|IP protocol for EYA1 antibody 22658-1-AP||Download protocol|
|Click here to view our Standard Protocols|
YTHDF1 phase separation triggers the fate transition of spermatogonial stem cells by activating the IκB-NF-κB-CCND1 axis
SIX1 Activates STAT3 Signaling to Promote the Proliferation of Thyroid Carcinoma via EYA1.
Eyes absent gene (EYA1) is a pathogenic driver and a therapeutic target for melanoma.
Stem Cell Res Ther
Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model.
Clin Breast Cancer
Dachshund 1 is Differentially Expressed Between Male and Female Breast Cancer: A Matched Case-Control Study of Clinical Characteristics and Prognosis.
J Mol Neurosci
MES23.5 DA Immortalized Neuroblastoma Cells Self-protect Against Early Injury by Overexpressing Glial Cell-derived Neurotrophic Factor via Akt1/Eya1/Six2 Signaling.