EYA3 Polyclonal antibody

EYA3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 21196-1-AP


EYA3, Eyes absent homolog 3

Tested Applications

Positive WB detected inHeLa cells, L02 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21196-1-AP is EYA3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EYA3 fusion protein Ag15544
Full Name eyes absent homolog 3 (Drosophila)
Calculated Molecular Weight 573 aa, 63 kDa
Observed Molecular Weight 63-66 kDa
GenBank Accession NumberBC029500
Gene Symbol EYA3
Gene ID (NCBI) 2140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EYA3(Eyes absent homolog 3) belongs to the HAD-like hydrolase superfamily.Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis.EYA3, like EYA1, is expressed in the ninth week of human development and may also underlie developmental defects(PMID:9020840).It has 2 isoforms produced by alternative splicing.This antibody is specific to EYA3.


Product Specific Protocols
WB protocol for EYA3 antibody 21196-1-APDownload protocol
IHC protocol for EYA3 antibody 21196-1-APDownload protocol
IF protocol for EYA3 antibody 21196-1-APDownload protocol
IP protocol for EYA3 antibody 21196-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

The EYA3 tyrosine phosphatase activity promotes pulmonary vascular remodeling in pulmonary arterial hypertension.

Authors - Yuhua Wang

Sci Rep

WDR1 is a novel EYA3 substrate and its dephosphorylation induces modifications of the cellular actin cytoskeleton.

Authors - Mihaela Mentel

Int J Mol Sci

Analysis of EYA3 Phosphorylation by Src Kinase Identifies Residues Involved in Cell Proliferation.

Authors - Aura E Ionescu

PLoS One

Structure-Activity Relationships of Benzbromarone Metabolites and Derivatives as EYA Inhibitory Anti-Angiogenic Agents.

Authors - Ram Naresh Pandey

Ann Transl Med

Both a hypoxia-inducible EYA3 and a histone acetyltransferase p300 function as coactivators of SIX5 to mediate tumorigenesis and cancer progression

Authors - Chunmei Yang

Mol Ther Nucleic Acids

miR-342-5p downstream to Notch enhances arterialization of endothelial cells in response to shear stress by repressing MYC

Authors - Xiaoyan Zhang