Anticorps Polyclonal de lapin anti-RHOA

• Phare
• Validé par KD/KO

RHOA Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

138

N° de cat : 10749-1-AP

Synonymes

ARH12, ARHA, h12, Rho cDNA clone 12, RHO12, RHOA, RHOH12, Transforming protein RhoA

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Galerie de données de validation

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  WB analysis using 10749-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10749-1-AP (RHOA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10749-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10749-1-AP (RHOA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 10749-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10749-1-AP (RHOA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10749-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10749-1-AP (RHOA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10749-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10749-1-AP (RHOA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10749-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10749-1-AP (RHOA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 10749-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RHOA antibody (10749-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa, cellules HUVEC, cellules MCF-7, cellules NIH/3T3, cellules PC-12, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain fœtal
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 126 publications below
• IHC: See 15 publications below
• IF: See 13 publications below

Informations sur le produit

10749-1-AP cible RHOA dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RHOA Protéine recombinante Ag1141
• Nom complet: ras homolog gene family, member A
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC005976
• Symbole du gène: RHOA
• Identification du gène (NCBI): 387
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

What is the function of RhoA?

Ras homolog gene family, member A (RhoA) is a small GTPase that is involved in cytoskeleton organization.¹ It is important in actomyosin contractility, as when RhoA binds to GTP it initiates a cascade of events mediating stress fiber or contractile ring formation, where actin bundles crosslink with other proteins. It is also associated with actin polymerization, making RhoA important in membrane ruffling and cell motility.² The role of RhoA is essential in cell migration and adhesion.

 What is the cellular localization of RhoA?

In motile cells, a leading edge stretches ahead, stabilizes, the cytoskeleton is reorganized, and the tail of the cell retracts. The role of RhoA in contracting actomyosin has linked RhoA with the tail retraction in this process, but studies into the spatiotemporal kinetics of GTPases have revealed that RhoA is also located at the leading edge of migrating cells.³ This highlights the dual roles of this protein in actin contractility and polymerization.

 What is the role of RhoA in disease?

Though it is not an oncogene, the role of RhoA in adhesion and migration has implicated this protein in cancer biology. Expression of RhoA has been found to be higher in malignant tumors compared to benign tumors or in non-tumor tissue, and more invasive tumors have been found to overexpress RhoA.⁴ This protein is also implicated in neurodegenerative diseases and aging due to its role in axon guidance, with abnormal localization of RhoA associated with Alzheimer's and Parkinson's pathology.^5,6 

1.     Ridley, A. J. & Hall, A. The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. Cell 70, 389-399 (1992).

2.     21.   Kurokawa, K. & Matsuda, M. Localized RhoA Activation as a Requirement for the Induction of Membrane Ruffling. Mol. Biol. Cell 16, 4294-4303 (2005).

3.     22.   Struckhoff, A. P., Rana, M. K. & Worthylake, R. A. RhoA can lead the way in tumor cell invasion and metastasis. Front. Biosci. (Landmark Ed. 16, 1915-26 (2011).

4.     23.   Mcglasson, S. et al. Rare variants of the 3'-5' DNA exonuclease in early onset TREX1 small vessel stroke [version 1; referees: awaiting peer review]. doi:10.12688/wellcomeopenres.12631.1

5.     24.   Huesa, G. et al. Altered Distribution of RhoA in Alzheimer's Disease and AβPP Overexpressing Mice. J. Alzheimer's Dis. 19, 37-56 (2010).

6.     25.   Hynds, D. L. Subcellular localization of Rho GTPases: implications for axon regeneration. Neural Regen. Res. 10, 1032-3 (2015).

Publications

Species	Application	Title
human	IF	Nat Commun; A mosquito salivary protein promotes flavivirus transmission by activation of autophagy.; Authors - Peng Sun
mouse	WB	Circ. Res.; Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.; Authors - Dong Ma
human	WB	Acta Biomater; Adipose-derived stem cells with miR-150-5p inhibition laden in hydroxyapatite/tricalcium phosphate ceramic powders promote osteogenesis via regulating Notch3 and activating FAK/ERK and RhoA; Authors - Fanglin Wang
mouse	WB	Autophagy; m6A mRNA methylation regulates testosterone synthesis through modulating autophagy in Leydig cells.; Authors - Yabing Chen
human	WB	Blood; Annexin II interactions with the annexin II receptor enhance multiple myeloma cell adhesion and growth in the bone marrow microenvironment.; Authors - Sonia D'Souza
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song

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