ROCK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ROCK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

93

Cat no : 21850-1-AP

Synonyms

Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, P160ROCK, p160 ROCK-1, p160 ROCK 1

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Validation Data Gallery

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  WB analysis using 21850-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21850-1-AP (ROCK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21850-1-AP

  WB result of ROCK1 antibody (21850-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK1 transfected HeLa cells.

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  IHC staining of human lymphoma using 21850-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21850-1-AP (ROCK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 21850-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21850-1-AP (ROCK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 21850-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ROCK1 antibody (21850-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HEK-293T cells, C6 cells, Jurkat cells, NIH/3T3 cells, Ramos cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 81 publications below
• IHC: See 13 publications below
• IF: See 10 publications below
• IP: See 1 publications below

Product Information

21850-1-AP targets ROCK1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ROCK1 fusion protein Ag16317
• Full Name: Rho-associated, coiled-coil containing protein kinase 1
• Calculated Molecular Weight: 1354 aa, 158 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC113114
• Gene Symbol: ROCK1
• Gene ID (NCBI): 6093
• RRID: AB_10953526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.; Authors - Li Wang
human	WB	Nat Commun; BioE3 identifies specific substrates of ubiquitin E3 ligases; Authors - Orhi Barroso-Gomila
mouse	IHC	Acta Biomater; Injectable self-assembling peptide nanofiber hydrogel as a bioactive 3D platform to promote chronic wound tissue regeneration.; Authors - Peng Lou
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
human,mouse	IHC,WB,IF	Biomed Pharmacother; Targeting ROCK1 in diabetic kidney disease: Unraveling mesangial fibrosis mechanisms and introducing myricetin as a novel antagonist; Authors - Ningning Yuan
rat	WB	Oxid Med Cell Longev; Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates.; Authors - Mengyang Zhang