CDC42 Polyclonal antibody

• Featured Product
• KD/KO Validated

CDC42 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

63

Reviews (2)

Cat no : 10155-1-AP

Synonyms

G25K GTP-binding protein, G25K GTP binding protein, G25K, EC:3.6.5.2

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Validation Data Gallery

• 

  WB analysis using 10155-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10155-1-AP (CDC42 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 10155-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10155-1-AP (CDC42 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10155-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10155-1-AP (CDC42 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 10155-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10155-1-AP (CDC42 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10155-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10155-1-AP (CDC42 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 10155-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10155-1-AP (CDC42 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 10155-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10155-1-AP (CDC42 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 10155-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10155-1-AP (CDC42 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 10155-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10155-1-AP (CDC42 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of C6 using 10155-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed C6 cells using 10155-1-AP (CDC42 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10155-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDC42 antibody (10155-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 10155-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10155-1-AP (CDC42 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HT-29 cells, C6 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue
• Positive IHC detected in: human pancreas cancer tissue, human pancreas tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 54 publications below
• IHC: See 6 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

10155-1-AP targets CDC42 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, bovine, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDC42 fusion protein Ag0207
• Full Name: cell division cycle 42 (GTP binding protein, 25kDa)
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC002711
• Gene Symbol: CDC42
• Gene ID (NCBI): 998
• RRID: AB_2078096
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cdc42, the human homologue of the cell division cycle 42 of Saccharomyces cerevisiae, belongs to the Rho/Rac subfamily of Ras-like GTPases. It acts as molecular switches to regulate numerous cellular responses including the organization of the actin cytoskeleton, gene transcription, cell cycle progression, and membrane trafficking. The molecular weight is about 21 kd.

Publications

Species	Application	Title
human	WB, IHC	Nat Commun; Spatiotemporal proteomic profiling of the pro-inflammatory response to lipopolysaccharide in the THP-1 human leukaemia cell line.; Authors - Claire M Mulvey
mouse	WB	Circ. Res.; Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.; Authors - Dong Ma
human	WB	Nucleic Acids Res; DOCK7 protects against replication stress by promoting RPA stability on chromatin.; Authors - Ming Gao
human	WB	Biomaterials; Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets.; Authors - Teng Zhou
mouse	IHC	Mol Ther Nucleic Acids; miR-497 defect contributes to gastric cancer tumorigenesis and progression via regulating CDC42/ITGB1/FAK/PXN/AKT signaling.; Authors - Lihui Zhang
mouse	WB	Commun Biol; RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling; Authors - Haixia Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-16-2023)

Worked well for WB and IF

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 for WB and 1:200 for IF
• Cell Tissue Type: Bone Marrow MSCs

FH

Ambra (Verified Customer) (01-19-2021)

I used it 1:500 in 5% milk in TBS-tween to have a nice signal in Human Brain lysate.The signal is quite low used 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Brain lysate (Temporal Cortex)

CDC42 Antibody Western Blot validation (1:500 dilution) in Human Brain lysate (Temporal Cortex) (Cat no:10155-1-AP)

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