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CDC42 Polyclonal antibody

CDC42 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (3)





Cat no : 10155-1-AP


CDC42, CDC42Hs, G25K, G25K GTP binding protein

Tested Applications

Positive WB detected inHT-29 cells, C6 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue, human pancreas tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC6 cells, HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10155-1-AP targets CDC42 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, hamster, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC42 fusion protein Ag0207
Full Name cell division cycle 42 (GTP binding protein, 25kDa)
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC002711
Gene Symbol CDC42
Gene ID (NCBI) 998
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cdc42, the human homologue of the cell division cycle 42 of Saccharomyces cerevisiae, belongs to the Rho/Rac subfamily of Ras-like GTPases. It acts as molecular switches to regulate numerous cellular responses including the organization of the actin cytoskeleton, gene transcription, cell cycle progression, and membrane trafficking. The molecular weight is about 21 kd.


Product Specific Protocols
WB protocol for CDC42 antibody 10155-1-APDownload protocol
IHC protocol for CDC42 antibody 10155-1-APDownload protocol
IF protocol for CDC42 antibody 10155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


humanWB, IHC

Nat Commun

Spatiotemporal proteomic profiling of the pro-inflammatory response to lipopolysaccharide in the THP-1 human leukaemia cell line.

Authors - Claire M Mulvey

Circ. Res.

Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.

Authors - Dong Ma

Nucleic Acids Res

DOCK7 protects against replication stress by promoting RPA stability on chromatin.

Authors - Ming Gao


Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets.

Authors - Teng Zhou

Mol Ther Nucleic Acids

miR-497 defect contributes to gastric cancer tumorigenesis and progression via regulating CDC42/ITGB1/FAK/PXN/AKT signaling.

Authors - Lihui Zhang

Commun Biol

RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling

Authors - Haixia Zhu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Udesh (Verified Customer) (08-16-2023)

Worked well for WB and IF

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:2000 for WB and 1:200 for IF
  • Cell Tissue Type: Bone Marrow MSCs

Ambra (Verified Customer) (01-19-2021)

I used it 1:500 in 5% milk in TBS-tween to have a nice signal in Human Brain lysate.The signal is quite low used 1:1000.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human Brain lysate (Temporal Cortex)
CDC42 Antibody Western Blot validation (1:500 dilution) in Human Brain lysate (Temporal Cortex) (Cat no:10155-1-AP)