Validation Data Gallery
|Positive WB detected in||HT-29 cells, HeLa cells, C6 cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue|
|Positive IHC detected in||human pancreas cancer tissue, human pancreas tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||C6 cells, HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10155-1-AP targets CDC42 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, chicken, hamster, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CDC42 fusion protein Ag0207|
|Full Name||cell division cycle 42 (GTP binding protein, 25kDa)|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||21 kDa|
|GenBank accession number||BC002711|
|Gene ID (NCBI)||998|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Cdc42, the human homologue of the cell division cycle 42 of Saccharomyces cerevisiae, belongs to the Rho/Rac subfamily of Ras-like GTPases. It acts as molecular switches to regulate numerous cellular responses including the organization of the actin cytoskeleton, gene transcription, cell cycle progression, and membrane trafficking. The molecular weight is about 21 kd.
Spatiotemporal proteomic profiling of the pro-inflammatory response to lipopolysaccharide in the THP-1 human leukaemia cell line.
Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.
Nucleic Acids Res
DOCK7 protects against replication stress by promoting RPA stability on chromatin.
Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets.
Mol Ther Nucleic Acids
miR-497 defect contributes to gastric cancer tumorigenesis and progression via regulating CDC42/ITGB1/FAK/PXN/AKT signaling.
RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Udesh (Verified Customer) (08-16-2023)
Worked well for WB and IF
Ambra (Verified Customer) (01-19-2021)
I used it 1:500 in 5% milk in TBS-tween to have a nice signal in Human Brain lysate.The signal is quite low used 1:1000.