RHOB Polyclonal antibody

• Featured Product
• KD/KO Validated

RHOB Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

26

Cat no : 14326-1-AP

Synonyms

ARH6, ARHB, h6, MST081, MSTP081, Rho cDNA clone 6, RHOB, RHOH6

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Validation Data Gallery

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  WB analysis using 14326-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14326-1-AP (RHOB antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14326-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14326-1-AP (RHOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14326-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14326-1-AP (RHOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 14326-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14326-1-AP (RHOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14326-1-AP

  IP result of anti-RHOB (IP:14326-1-AP, 3ug; Detection:14326-1-AP 1:2000) with HeLa cells lysate 2800ug.

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  IP experiment of mouse brain using 14326-1-AP

  IP result of anti-RHOB (IP:14326-1-AP, 4ug; Detection:14326-1-AP 1:1000) with mouse brain tissue lysate 4400 ug.

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  IHC staining of human brain using 14326-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14326-1-AP (RHOB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14326-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14326-1-AP (RHOB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293 cells, HeLa cells, SH-SY5Y cells, rat brain tissue
• Positive IP detected in: HeLa cells, mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 25 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

14326-1-AP targets RHOB in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RHOB fusion protein Ag5593
• Full Name: ras homolog gene family, member B
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC066954
• Gene Symbol: RHOB
• Gene ID (NCBI): 388
• RRID: AB_2179092
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Mol Cancer; Hsa-miR-3178/RhoB/PI3K/Akt, a novel signaling pathway regulates ABC transporters to reverse gemcitabine resistance in pancreatic cancer.; Authors - Jianyou Gu
human	WB,IF	Nat Commun; ATR/Chk1 signaling induces autophagy through sumoylated RhoB-mediated lysosomal translocation of TSC2 after DNA damage.; Authors - Mingdong Liu ; KO Validated
human	WB	J Med Chem; Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.; Authors - Ting Peng
human	WB	Mol Cancer Ther; Expression signatures of the lipid-based Akt inhibitors phosphatidylinositol ether lipid analogues in NSCLC cells.; Authors - Zhang Chunyu C ; KD Validated
rat	WB	Sci Rep; MicroRNA-223 Attenuates Hypoxia-induced Vascular Remodeling by Targeting RhoB/MLC2 in Pulmonary Arterial Smooth Muscle Cells.; Authors - Yan Zeng
	WB	J Cell Biochem; Androgen receptor activation decreases proliferation in thyroid cancer cells.; Authors - Melanie E Jones

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