Tested Applications
| Positive WB detected in | HL-60 cells, human brain tissue, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human lung cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24072-1-AP targets RAC1 in WB, IHC, IF/ICC, IP, coIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17779 Product name: Recombinant human RAC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-192 aa of BC004247 Sequence: MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLIPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL Predict reactive species |
| Full Name | ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC004247 |
| Gene Symbol | RAC1 |
| Gene ID (NCBI) | 5879 |
| RRID | AB_2879427 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P63000 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAC1 antibody 24072-1-AP | Download protocol |
| IHC protocol for RAC1 antibody 24072-1-AP | Download protocol |
| WB protocol for RAC1 antibody 24072-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAC1 Polyclonal antibody (Cat# 24072-1-AP) has 145 citations. They appear in journals including Cell, Nature Cancer, Nature Metabolism, Nature Communications, Gastroenterology, Cancer Research, Kidney International, Cell Death & Differentiation, Cell Reports, Science Advances, and Oncogene. Research fields span oncology (hepatocellular, lung, ovarian, colorectal, and other cancers), cell migration and cytoskeleton dynamics, Rho GTPase signaling, immunology, inflammation, fibrosis, neuroscience, and metabolic disease.
| Species | Application | Title |
|---|---|---|
Gastroenterology Cell-in-Cell-Mediated Entosis Reveals A Progressive Mechanism in Pancreatic Cancer | ||
Nat Cancer Cardiomyocyte-localized CCDC25 senses NET DNA to promote doxorubicin cardiotoxicity by activating autophagic flux | ||
Nat Metab Metabolic enzyme LDHA activates Rac1 GTPase as a noncanonical mechanism to promote cancer | ||
Bioact Mater Bioactive vascular buds promote collateral vessel formation by grafting on the artificial vessel walls |
