ROCK2-Specific(C-term) Polyclonal antibody

• Featured Product
• KD/KO Validated

ROCK2-Specific(C-term) Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 20248-1-AP

Synonyms

KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2

---

Validation Data Gallery

• 

  WB analysis of HeLa using 20248-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20248-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20248-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 20248-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 20248-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 20248-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 20248-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 20248-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 20248-1-AP

  Immunofluorescent analysis of HepG2 cells, using ROCK2 antibody 20248-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human colon tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Product Information

20248-1-AP targets ROCK2-Specific(C-term) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Rho-associated, coiled-coil containing protein kinase 2
• Calculated Molecular Weight: 161 kDa
• Observed Molecular Weight: 164 kDa
• GenBank Accession Number: NM_004850
• Gene Symbol: ROCK2
• Gene ID (NCBI): 9475
• RRID: AB_10665551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.

Publications

Species	Application	Title
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
human	WB,IHC	Cell Prolif; The distinct roles of myosin IIA and IIB under compression stress in nucleus pulposus cells.; Authors - Wencan Ke
human	WB,IHC,IF	Exp Neurol; PMID: 27637803; Authors - Jiong Yue
human	WB	Chin J Nat Med; Stigmasterol protects human brain microvessel endothelial cells against ischemia-reperfusion injury through suppressing EPHA2 phosphorylation; Authors - Suping Li
rat	IHC	Oxid Med Cell Longev; Lycopene Ameliorates Transplant Arteriosclerosis in Vascular Allograft Transplantation by Regulating the NO/cGMP Pathways and Rho-Associated Kinases Expression.; Authors - Yunqiang He
mouse	WB	ASN Neuro; Induction of oligodendrocyte differentiation and in vitro myelination by inhibition of rho-associated kinase.; Authors - Carlos E Pedraza ; KD Validated