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  • KD/KO Validated

ROCK2-Specific(C-term) Polyclonal antibody

ROCK2-Specific(C-term) Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 20248-1-AP


KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20248-1-AP targets ROCK2-Specific(C-term) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Rho-associated, coiled-coil containing protein kinase 2
Calculated Molecular Weight 161 kDa
Observed Molecular Weight 164 kDa
GenBank Accession NumberNM_004850
Gene Symbol ROCK2
Gene ID (NCBI) 9475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.


Product Specific Protocols
WB protocol for ROCK2-Specific(C-term) antibody 20248-1-APDownload protocol
IHC protocol for ROCK2-Specific(C-term) antibody 20248-1-APDownload protocol
IF protocol for ROCK2-Specific(C-term) antibody 20248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Authors - Hongyuan Song

Cell Prolif

The distinct roles of myosin IIA and IIB under compression stress in nucleus pulposus cells.

Authors - Wencan Ke

Exp Neurol

PMID: 27637803

Authors - Jiong Yue

Chin J Nat Med

Stigmasterol protects human brain microvessel endothelial cells against ischemia-reperfusion injury through suppressing EPHA2 phosphorylation

Authors - Suping Li

Oxid Med Cell Longev

Lycopene Ameliorates Transplant Arteriosclerosis in Vascular Allograft Transplantation by Regulating the NO/cGMP Pathways and Rho-Associated Kinases Expression.

Authors - Yunqiang He

ASN Neuro

Induction of oligodendrocyte differentiation and in vitro myelination by inhibition of rho-associated kinase.

Authors - Carlos E Pedraza
  • KD Validated