Anticorps Polyclonal de lapin anti-REDD1 specific

• Phare
• Validé par KD/KO

REDD1 specific Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (5)

Applications

WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA

Conjugaison

Non conjugué

Publications

263

Avis (4)

N° de cat : 10638-1-AP

Synonymes

DDIT4, Dig2, REDD 1, REDD1, REDD1 specific, RP11 442H21.1, RTP801

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Galerie de données de validation

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  WB analysis using 10638-1-AP

  Non-treated Hela and Cobalt Chloride treated Hela cells were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 incubated at room temperature for 6 hours. .

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  WB analysis of PC-3 cells using 10638-1-AP

  WB result of REDD1 antibody (10638-1-AP, 1:1000) with si-control and si-REDD1 transfected PC-3 cells.

• 

  WB analysis using 10638-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10638-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10638-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 10638-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10638-1-AP

  IP result of anti-REDD1 specific (IP:10638-1-AP, 3ug; Detection:10638-1-AP 1:500) with MCF-7 cells lysate 2500ug.

• 

  IHC staining of human lung cancer using 10638-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10638-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10638-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10638-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10638-1-AP (REDD1 specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 10638-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10638-1-AP (REDD1 specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 10638-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10638-1-AP (REDD1 specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 10638-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10638-1-AP (REDD1 specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of LNCaP using 10638-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using REDD1 specific antibody (10638-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules DU 145, cellules HeLa traitées au chlorure de cobalt, cellules K-562, cellules LNCaP, cellules PC-3
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cardiaque humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules LNCaP,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 38 publications below
• WB: See 245 publications below
• IHC: See 23 publications below
• IF: See 16 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below

Informations sur le produit

10638-1-AP cible REDD1 specific dans les applications de WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, Lapin, porc, souris, Gerbille, squirrel
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: REDD1 specific Protéine recombinante Ag0965
• Nom complet: DNA-damage-inducible transcript 4
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 32-35 kDa
• Numéro d’acquisition GenBank: BC007714
• Symbole du gène: REDD1/DDIT4
• Identification du gène (NCBI): 54541
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489]. This antibody is a rabbit polyclonal antibody raised against full length human REDD1 antigen. This antibody is specific to the REDD1 from siRNA experiment (PMID:24713927)

Publications

Species	Application	Title
mouse	WB	Cell; Plzf regulates germline progenitor self-renewal by opposing mTORC1.; Authors - Hobbs Robin M RM
human	WB	Cell; Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR.; Authors - Zandra E Walton
mouse	WB	Cancer Cell; A UBE2O-AMPKα2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy.; Authors - Isabelle K Vila
rat	WB	Nat Med; REDD1 is essential for stress-induced synaptic loss and depressive behavior.; Authors - Kristie T Ota
mouse	chIP	Nat Immunol; The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity.; Authors - Wilson Castro
mouse	WB	Acta Neuropathol; MEF2 impairment underlies skeletal muscle atrophy in polyglutamine disease.; Authors - Samir R Nath

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yuki (Verified Customer) (11-10-2022)

This product was used for IHC in human tissue.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: kidney

REDD1 specific Antibody Immunohistochemistry validation (1:50 dilution) in kidney (Cat no:10638-1-AP)

FH

Stephane (Verified Customer) (11-26-2020)

The antibody performs great on human cell extract. It detects specific bands with the REDD1 band resolving around 30-35 kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

REDD1 specific Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:10638-1-AP)

FH

Jamal (Verified Customer) (09-17-2019)

This product was used for Western Blotting in mouse cortex tissue.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse Brain Tissue

FH

Bradley (Verified Customer) (08-19-2019)

I have used this antibody on numerous occasions with success. The antibody performs great on human cell extract, as evidenced by a single immunoreactive band. The antibody performs moderately well on mouse skeletal muscle extract as it detects many non-specific bands with the REDD1 band resolving around 32-35 kD (specificity determined using REDD1-/- samples). The antibody is capable of detecting large changes in REDD1 protein in mouse skeletal muscle (i.e. induction following dexamethasone treatment), but smaller changes are difficult to assess in this tissue. This antibody will detect smaller changes in human cell lines such as HEK 293 or Hela cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500 for mouse skeletal muscle. 1:1000 for human cells
• Cell Tissue Type: Mouse skeletal muscle. HEK293 cells

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