Hamartin/TSC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Hamartin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 20988-1-AP

Synonyms

Hamartin, KIAA0243, LAM, TSC, TSC1, tuberous sclerosis 1, Tuberous sclerosis 1 protein

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Validation Data Gallery

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  WB analysis of HeLa using 20988-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20988-1-AP (hamartin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20988-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20988-1-AP (hamartin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 20988-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 20988-1-AP (hamartin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 20988-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 20988-1-AP (hamartin antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

20988-1-AP targets Hamartin in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tuberous sclerosis 1
• Calculated Molecular Weight: 130 kDa
• GenBank Accession Number: NM_000368
• Gene Symbol: Hamartin/TSC1
• Gene ID (NCBI): 7248
• RRID: AB_10950398
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSC1, also named as KIAA0243 and TSC, is implicated as a tumor suppressor. It is involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. In complex with TSC2, TSC1 inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. It seems not to be required for TSC2 GAP activity towards RHEB. It is involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Defects in TSC1 are the cause of tuberous sclerosis complex (TSC). Defects in TSC1 may be a cause of focal cortical dysplasia of Taylor balloon cell type (FCDBC). The antibody is specific to TSC1. This antibody can recognize two isoforms: 70 kDa (667aa) and 130 kDa.

Publications

Species	Application	Title
human	WB	Sci Adv; Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.; Authors - Seung Ho Shin
mouse	WB	Theranostics; Combination therapy with ropivacaine-loaded liposomes and nutrient deprivation for simultaneous cancer therapy and cancer pain relief.; Authors - Jiqian Zhang ; KD Validated
human	WB	Mol Ther Nucleic Acids; miR-301a Suppression within Fibroblasts Limits the Progression of Fibrosis through the TSC1/mTOR Pathway.; Authors - Jiexuan Wang ; KD Validated
mouse	WB	Sci Rep; Tsc1 deficiency impairs mammary development in mice by suppression of AKT, nuclear ERα, and cell-cycle-driving proteins.; Authors - Zhenqi Qin ; KO Validated
mouse	WB	FASEB J; mTORC1 promotes mineralization via p53 pathway.; Authors - Xinghong Luo
human	WB,IHC,IP	Biochem Biophys Res Commun; UBE2S exerts oncogenic activities in urinary bladder cancer by ubiquitinating TSC1.; Authors - Hao Tang ; KD Validated