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- KD/KO Validated
p70(S6K) Polyclonal antibody
p70(S6K) Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 14485-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, IGF-1 treated MCF-7 cells, Jurkat cells, NIH/3T3 cells, HeLa cells, PC-12 cells, C6 cells, mouse brain tissue, rat brain tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
14485-1-AP targets p70(S6K) in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, rabbit, pig, Megalobrama amblycephala|
|Host / Isotype||Rabbit / IgG|
|Immunogen||p70(S6K) fusion protein Ag5883|
|Full Name||ribosomal protein S6 kinase, 70kDa, polypeptide 1|
|Calculated molecular weight||59 kDa|
|Observed molecular weight||65-75 kDa, 80-85 kDa|
|GenBank accession number||BC053365|
|Gene ID (NCBI)||6198|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA, and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of mTOR and acts as a crucial effector of mTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K). The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation, and metabolism and is the central regulator of translation of some components of the protein synthesis system. Due to alternative translation, two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1).
|Product Specific Protocols|
|WB protocol for p70(S6K) antibody 14485-1-AP||Download protocol|
|IHC protocol for p70(S6K) antibody 14485-1-AP||Download protocol|
|IF protocol for p70(S6K) antibody 14485-1-AP||Download protocol|
|IP protocol for p70(S6K) antibody 14485-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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