mTOR Polyclonal antibody

• Featured Product
• KD/KO Validated

mTOR Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

199

Cat no : 20657-1-AP

Synonyms

FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1

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Validation Data Gallery

• 

  WB analysis of MCF-7 using 20657-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20657-1-AP (MTOR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 20657-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20657-1-AP (MTOR Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20657-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20657-1-AP (MTOR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 20657-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20657-1-AP (mTOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20657-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20657-1-AP (mTOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20657-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20657-1-AP (mTOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20657-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20657-1-AP (mTOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 20657-1-AP

  Immunofluorescent analysis of HeLa cells using 20657-1-AP (MTOR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, MDA-MB-453s cells, HeLa cells
• Positive IHC detected in: mouse testis tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 188 publications below
• IHC: See 8 publications below
• IF: See 9 publications below

Product Information

20657-1-AP targets mTOR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine, chicken, bovine, hamster, fish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: FK506 binding protein 12-rapamycin associated protein 1
• Calculated Molecular Weight: 289 kDa
• GenBank Accession Number: NM_004958
• Gene Symbol: mTOR
• Gene ID (NCBI): 2475
• RRID: AB_10734440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359). The antibody is specific to MTOR.

Publications

Species	Application	Title
human	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu
mouse	WB	J Exp Med; Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.; Authors - Zhe Chen
human	WB	Cell Death Differ; PIWIL2 interacting with IKK to regulate autophagy and apoptosis in esophageal squamous cell carcinoma.; Authors - Xu Zhao
human	WB	Autophagy; FAIM regulates autophagy through glutaminolysis in lung adenocarcinoma.; Authors - Tianyu Han
rat	IF	Autophagy; Autophagy and post-ischemic conditioning in retinal ischemia.; Authors - Biji Mathew
human	WB	Int J Biol Macromol; Novel soybean polypeptide dglycin alleviates atherosclerosis in apolipoprotein E-deficient mice; Authors - Han Zhao

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